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Allele Frequency of Resistance to Bacillus thuringiensis Cry1Ab Corn in Louisiana Populations of Sugarcane Borer (Lepidoptera: Crambidae)
Transgenic Bacillus thuringiensis (Bt) corn, Zea mays L., has been widely used to manage a corn borer complex in the mid-southern region of the United States. The sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), has become a dominant cornstalk boring species in some areas of this...
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| Published in: | Journal of economic entomology 2008-04, Vol.101 (2), p.492-498 |
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| container_end_page | 498 |
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| container_title | Journal of economic entomology |
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| creator | Huang, Fangneng Leonard, B. Rogers Moore, Steven H Cook, Donald R Baldwin, Jack Tindall, Kelly V Lee, Donna R |
| description | Transgenic Bacillus thuringiensis (Bt) corn, Zea mays L., has been widely used to manage a corn borer complex in the mid-southern region of the United States. The sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in Louisiana. Therefore, management of sugarcane borer resistance to Bt corn is critical to ensure the long-term sustainability of Bt corn for the region. This study screened 280 two-parent family-lines of sugarcane borer from four geographical populations in Louisiana during 2005 to determine whether Bt resistance allele frequency in sugarcane borer is sufficiently low to meet the rare resistance assumption of the current “high dose/refuge” resistance management strategy for Bt corn. These sugarcane borer family-lines were examined for Bt resistance by using novel F2 screening procedures. No major Bt resistance alleles were detected in these four populations. The estimated frequency of major Bt resistance alleles was |
| doi_str_mv | 10.1603/0022-0493%282008%29101%5B492%3AAFORTB%5D2.0.CO%3B2 |
| format | article |
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Rogers ; Moore, Steven H ; Cook, Donald R ; Baldwin, Jack ; Tindall, Kelly V ; Lee, Donna R</creator><creatorcontrib>Huang, Fangneng ; Leonard, B. Rogers ; Moore, Steven H ; Cook, Donald R ; Baldwin, Jack ; Tindall, Kelly V ; Lee, Donna R</creatorcontrib><description>Transgenic Bacillus thuringiensis (Bt) corn, Zea mays L., has been widely used to manage a corn borer complex in the mid-southern region of the United States. The sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in Louisiana. Therefore, management of sugarcane borer resistance to Bt corn is critical to ensure the long-term sustainability of Bt corn for the region. This study screened 280 two-parent family-lines of sugarcane borer from four geographical populations in Louisiana during 2005 to determine whether Bt resistance allele frequency in sugarcane borer is sufficiently low to meet the rare resistance assumption of the current “high dose/refuge” resistance management strategy for Bt corn. These sugarcane borer family-lines were examined for Bt resistance by using novel F2 screening procedures. No major Bt resistance alleles were detected in these four populations. The estimated frequency of major Bt resistance alleles was <0.0027, with a 95% probability and a detection power of 94%. The estimated minor resistance allele frequency was 0.0063, with a 95% CI of 0.0025–0.0117. During a previous study, a major Bt resistance allele was detected in one individual from 213 family-lines of another Louisiana population of sugarcane borer. Combining these data with the current screen, the frequency of major Bt resistance alleles across the five populations was 0.001, with a 95% credibility interval of 0.0001–0.0028 and a detection power of 95%. Major Bt resistance allele frequencies in Louisiana sugarcane borer populations seem to be low, and they should support the rare resistance allele requirement of the high dose/refuge strategy.</description><identifier>ISSN: 0022-0493</identifier><identifier>EISSN: 1938-291X</identifier><identifier>DOI: 10.1603/0022-0493%282008%29101%5B492%3AAFORTB%5D2.0.CO%3B2</identifier><identifier>PMID: 18459416</identifier><language>eng</language><publisher>England: Entomological Society of America</publisher><subject>Alleles ; Animals ; Bacillus thuringiensis ; Bacterial Proteins - genetics ; Bacterial Proteins - pharmacology ; Bt corn ; Bt resistance ; corn ; cornstalk borers ; crystal proteins ; Diatraea saccharalis ; Endotoxins - genetics ; Endotoxins - pharmacology ; gene frequency ; genetic resistance ; germplasm screening ; Hemolysin Proteins - genetics ; Hemolysin Proteins - pharmacology ; insecticide resistance ; Insecticide Resistance - genetics ; INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT ; insecticide resistance management ; Louisiana ; monitoring ; Moths - drug effects ; Moths - genetics ; Plants, Genetically Modified ; population genetics ; resistance alleles ; resistance management ; resistance monitoring ; transgenic plants ; Zea mays ; Zea mays - genetics</subject><ispartof>Journal of economic entomology, 2008-04, Vol.101 (2), p.492-498</ispartof><rights>Entomological Society of America</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18459416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Fangneng</creatorcontrib><creatorcontrib>Leonard, B. Rogers</creatorcontrib><creatorcontrib>Moore, Steven H</creatorcontrib><creatorcontrib>Cook, Donald R</creatorcontrib><creatorcontrib>Baldwin, Jack</creatorcontrib><creatorcontrib>Tindall, Kelly V</creatorcontrib><creatorcontrib>Lee, Donna R</creatorcontrib><title>Allele Frequency of Resistance to Bacillus thuringiensis Cry1Ab Corn in Louisiana Populations of Sugarcane Borer (Lepidoptera: Crambidae)</title><title>Journal of economic entomology</title><addtitle>J Econ Entomol</addtitle><description>Transgenic Bacillus thuringiensis (Bt) corn, Zea mays L., has been widely used to manage a corn borer complex in the mid-southern region of the United States. The sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), has become a dominant cornstalk boring species in some areas of this region, especially in Louisiana. Therefore, management of sugarcane borer resistance to Bt corn is critical to ensure the long-term sustainability of Bt corn for the region. This study screened 280 two-parent family-lines of sugarcane borer from four geographical populations in Louisiana during 2005 to determine whether Bt resistance allele frequency in sugarcane borer is sufficiently low to meet the rare resistance assumption of the current “high dose/refuge” resistance management strategy for Bt corn. These sugarcane borer family-lines were examined for Bt resistance by using novel F2 screening procedures. No major Bt resistance alleles were detected in these four populations. The estimated frequency of major Bt resistance alleles was <0.0027, with a 95% probability and a detection power of 94%. The estimated minor resistance allele frequency was 0.0063, with a 95% CI of 0.0025–0.0117. During a previous study, a major Bt resistance allele was detected in one individual from 213 family-lines of another Louisiana population of sugarcane borer. Combining these data with the current screen, the frequency of major Bt resistance alleles across the five populations was 0.001, with a 95% credibility interval of 0.0001–0.0028 and a detection power of 95%. Major Bt resistance allele frequencies in Louisiana sugarcane borer populations seem to be low, and they should support the rare resistance allele requirement of the high dose/refuge strategy.</description><subject>Alleles</subject><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bt corn</subject><subject>Bt resistance</subject><subject>corn</subject><subject>cornstalk borers</subject><subject>crystal proteins</subject><subject>Diatraea saccharalis</subject><subject>Endotoxins - genetics</subject><subject>Endotoxins - pharmacology</subject><subject>gene frequency</subject><subject>genetic resistance</subject><subject>germplasm screening</subject><subject>Hemolysin Proteins - genetics</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT</subject><subject>insecticide resistance management</subject><subject>Louisiana</subject><subject>monitoring</subject><subject>Moths - drug effects</subject><subject>Moths - genetics</subject><subject>Plants, Genetically Modified</subject><subject>population genetics</subject><subject>resistance alleles</subject><subject>resistance management</subject><subject>resistance monitoring</subject><subject>transgenic plants</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><issn>0022-0493</issn><issn>1938-291X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpFkctu1DAUQC0EokPhF8CbIFhk8Ctp3F0SGEAaaao-JHbWTeIMRhk72MliPqF_XafTwsLywkdH1_cgdEnJmuaEfyGEsZQIyRNWMEKKhElKaJJVQrKEl-Vmd31bJdlXtibrepfwir1AKyp5kUbw10u0-ic4Q29C-EMIzRklr9EZLUQmBc1X6L4cBj1ovPH676xte8Sux9c6mDCBbTWeHK6gNcMwBzz9nr2xe6NtfMa1P9KywbXzFhuLt242wYAFfOXGeYDJOBsW2c28B9-C1bhyXnv8aatH07lx0h4uowUOjelAf36LXvUwBP3u6T5Hd5tvt_WPdLv7_rMut2lDM5qnUtK872UcX_bQsLbLedcwASKejra8aQCEkKCzjJG-6BksQC5EqzOZk46fo48n7-hd_HKY1MGEVg9DHNHNQeWSSlaQiwi-fwLn5qA7NXpzAH9Uz8uLwIcT0INTsPcmqLsbRiiPrSTjFzQSVyeiMc5Z_V9B1FJYLYHUEkidCqvHwuqxsHourGJhRVS9U7EwfwCYw5ov</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Huang, Fangneng</creator><creator>Leonard, B. Rogers</creator><creator>Moore, Steven H</creator><creator>Cook, Donald R</creator><creator>Baldwin, Jack</creator><creator>Tindall, Kelly V</creator><creator>Lee, Donna R</creator><general>Entomological Society of America</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Allele Frequency of Resistance to Bacillus thuringiensis Cry1Ab Corn in Louisiana Populations of Sugarcane Borer (Lepidoptera: Crambidae)</title><author>Huang, Fangneng ; Leonard, B. Rogers ; Moore, Steven H ; Cook, Donald R ; Baldwin, Jack ; Tindall, Kelly V ; Lee, Donna R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b1516-9916ff95949fab2cd63db24a424ad1c3bbaa449ae5520f8f2a63db644ce5960d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Bacillus thuringiensis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bt corn</topic><topic>Bt resistance</topic><topic>corn</topic><topic>cornstalk borers</topic><topic>crystal proteins</topic><topic>Diatraea saccharalis</topic><topic>Endotoxins - genetics</topic><topic>Endotoxins - pharmacology</topic><topic>gene frequency</topic><topic>genetic resistance</topic><topic>germplasm screening</topic><topic>Hemolysin Proteins - genetics</topic><topic>Hemolysin Proteins - pharmacology</topic><topic>insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT</topic><topic>insecticide resistance management</topic><topic>Louisiana</topic><topic>monitoring</topic><topic>Moths - drug effects</topic><topic>Moths - genetics</topic><topic>Plants, Genetically Modified</topic><topic>population genetics</topic><topic>resistance alleles</topic><topic>resistance management</topic><topic>resistance monitoring</topic><topic>transgenic plants</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Fangneng</creatorcontrib><creatorcontrib>Leonard, B. Rogers</creatorcontrib><creatorcontrib>Moore, Steven H</creatorcontrib><creatorcontrib>Cook, Donald R</creatorcontrib><creatorcontrib>Baldwin, Jack</creatorcontrib><creatorcontrib>Tindall, Kelly V</creatorcontrib><creatorcontrib>Lee, Donna R</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of economic entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Fangneng</au><au>Leonard, B. 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Therefore, management of sugarcane borer resistance to Bt corn is critical to ensure the long-term sustainability of Bt corn for the region. This study screened 280 two-parent family-lines of sugarcane borer from four geographical populations in Louisiana during 2005 to determine whether Bt resistance allele frequency in sugarcane borer is sufficiently low to meet the rare resistance assumption of the current “high dose/refuge” resistance management strategy for Bt corn. These sugarcane borer family-lines were examined for Bt resistance by using novel F2 screening procedures. No major Bt resistance alleles were detected in these four populations. The estimated frequency of major Bt resistance alleles was <0.0027, with a 95% probability and a detection power of 94%. The estimated minor resistance allele frequency was 0.0063, with a 95% CI of 0.0025–0.0117. During a previous study, a major Bt resistance allele was detected in one individual from 213 family-lines of another Louisiana population of sugarcane borer. Combining these data with the current screen, the frequency of major Bt resistance alleles across the five populations was 0.001, with a 95% credibility interval of 0.0001–0.0028 and a detection power of 95%. Major Bt resistance allele frequencies in Louisiana sugarcane borer populations seem to be low, and they should support the rare resistance allele requirement of the high dose/refuge strategy.</abstract><cop>England</cop><pub>Entomological Society of America</pub><pmid>18459416</pmid><doi>10.1603/0022-0493%282008%29101%5B492%3AAFORTB%5D2.0.CO%3B2</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of economic entomology, 2008-04, Vol.101 (2), p.492-498 |
| issn | 0022-0493 1938-291X |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Alleles Animals Bacillus thuringiensis Bacterial Proteins - genetics Bacterial Proteins - pharmacology Bt corn Bt resistance corn cornstalk borers crystal proteins Diatraea saccharalis Endotoxins - genetics Endotoxins - pharmacology gene frequency genetic resistance germplasm screening Hemolysin Proteins - genetics Hemolysin Proteins - pharmacology insecticide resistance Insecticide Resistance - genetics INSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT insecticide resistance management Louisiana monitoring Moths - drug effects Moths - genetics Plants, Genetically Modified population genetics resistance alleles resistance management resistance monitoring transgenic plants Zea mays Zea mays - genetics |
| title | Allele Frequency of Resistance to Bacillus thuringiensis Cry1Ab Corn in Louisiana Populations of Sugarcane Borer (Lepidoptera: Crambidae) |
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