Delay expression of limonoid UDP-glucosyltransferase makes delayed bitterness in citrus

Genes encoding limonoid UDP-glucosyltransferase from albedo of six Citrus species with different levels of delayed bitterness are isolated and cloned in vector pTZ57R/T. Our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with Satsuma...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-06, Vol.371 (1), p.59-62
Main Authors: Zaare-Nahandi, Fariborz, Hosseinkhani, Saman, Zamani, Zabihollah, Asadi-Abkenar, Asad, Omidbaigi, Reza
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Amino Acid Sequence
Beverages
Citrus
Citrus - enzymology
Citrus - genetics
Citrus - growth & development
Delayed bitterness
Gene expression
Gene Expression Regulation, Plant
Glucosyltransferases - classification
Glucosyltransferases - genetics
Limonoid UDP-glucosyltransferase
Molecular Sequence Data
Phylogeny
Plant Proteins - classification
Plant Proteins - genetics
Protein Structure, Tertiary
Sequence Analysis, DNA
Taste
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genes encoding limonoid UDP-glucosyltransferase from albedo of six Citrus species with different levels of delayed bitterness are isolated and cloned in vector pTZ57R/T. Our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with Satsuma mandarin (without distinctive juice delayed bitterness). Also gene sequence of Marsh seedless grapefruit, local orange and Thompson navel orange (with mild juice delayed bitterness) have very similarity with Satsuma mandarin. On the other hand, this gene started to express 60, 120, and 210 days after full blooming in albedo of Satsuma mandarin, sweet oranges and sour orange, and both grapefruit and sweet lime, respectively. Expression pattern of limonoid glucosyltransferase gene in leaves was quite different with albedo. Thus, we supposed the delayed bitterness in this species was related to delay in expression of limonoid glucosyltransferase gene in albedo and lower limonoid glucoside accumulation in fruits.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.03.157