Bovine hemoglobin: An attractive source of antibacterial peptides

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2008-06, Vol.29 (6), p.969-977
• Main Authors: Nedjar-Arroume, Naïma, Dubois-Delval, Véronique, Adje, Estelle Yaba, Traisnel, Jonathan, Krier, François, Mary, Patrice, Kouach, Mostafa, Briand, Gilbert, Guillochon, Didier
• Format: Article
• Language: English
• Subjects: Active peptides; Amino Acid Sequence; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial peptides; Biological and medical sciences; Bovine hemoglobin; Cattle; Chromatography, High Pressure Liquid; Escherichia coli - drug effects; Fundamental and applied biological sciences. Psychology; Hemoglobins - chemistry; Hemoglobins - pharmacology; Hydrogen-Ion Concentration; Hydrolysate; Hydrolysis; Hydrophobic and Hydrophilic Interactions; Listeria - drug effects; Mass Spectrometry; Microbial Sensitivity Tests; Micrococcus luteus - drug effects; Molecular Sequence Data; Pepsin; Pepsin A - pharmacology; Peptide Fragments - analysis; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Peptide Fragments - pharmacology; Peptides - chemistry; Peptides - pharmacology; Protein Structure, Secondary; Salmonella enteritidis - drug effects; Vertebrates: endocrinology
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2008.01.011

A peptic hemoglobin hydrolysate was fractioned by a semi-preparative reversed-phase HPLC and some fractions have an antibacterial activity against four bacteria strains: Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis. These fractions were analyzed by ESI/MS and ESI/MS/MS, in order to characterize the peptides in these fractions. Each fraction contains at least three peptides and some fractions contain five peptides. All these fractions were purified several times by HPLC to obtain pure peptides. Thirty antibacterial peptides were identified. From the isolated antibacterial peptides, 24 peptides were derived from the α chains of hemoglobin and 6 peptides were derived from the β chains of hemoglobin. The lowest concentration of these peptides (minimum inhibitory concentration (MIC)) necessary to completely inhibit the growth of four bacteria strain was determined. The cell population of all of the tested bacteria species decreased by at least 97% after a 24-h incubation with any of the peptides at the minimum inhibitory concentration.