Modified expression of cytoplasmic isocitrate dehydrogenase electrophoretic isoforms in seminal plasma of men with sertoli-cell-only syndrome and seminoma

Two isoforms of human cytoplasmic isocitrate dehydrogenase (IDPc) of close molecular weights and different isoelectric points were identified in human seminal plasma (SP) by two‐dimensional gel electrophoresis (2‐DE) followed by mass spectrometry (MS). These two isoforms were detected in the normosp...

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Bibliographic Details
Published in:Molecular carcinogenesis 2008-06, Vol.47 (6), p.410-414
Main Authors: Starita-Geribaldi, Mireille, Samson, Michel, Guigonis, Jean-Marie, Pointis, Georges, Fenichel, Patrick
Format: Article
Language:English
Subjects:
Cytoplasm - enzymology
cytoplasmic isocitrate dehydrogenase
Electrophoresis, Gel, Two-Dimensional - methods
Humans
Isocitrate Dehydrogenase - isolation & purification
Isocitrate Dehydrogenase - metabolism
Male
mass spectrometry
Semen - enzymology
Seminoma - enzymology
Sertoli Cell-Only Syndrome - enzymology
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
spermatogenesis impairment
Testicular Neoplasms - enzymology
testicular seminoma
two-dimensional gel electrophoresis
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Summary:Two isoforms of human cytoplasmic isocitrate dehydrogenase (IDPc) of close molecular weights and different isoelectric points were identified in human seminal plasma (SP) by two‐dimensional gel electrophoresis (2‐DE) followed by mass spectrometry (MS). These two isoforms were detected in the normospermic men SP and their expressions were markedly altered in patients with testicular seminoma, the most frequent testicular germ cell cancer (TGCC): increase of the more acidic spot and decrease of the more basic one. Since oligospermia has been considered as a high risk pathological condition for developing a testicular cancer, the two IDPc isoforms were analyzed in SP of a group of secretory azoospermic patients. In this group the two spots displayed similar variations of expression to those observed in testicular seminoma. These results propose IDPc as a promising SP biomarker of testicular seminoma. Whether IDPc alteration in secretory azoospermia is predictive of testicular seminoma remains to be elucidated. © 2007 Wiley‐Liss, Inc.
ISSN:0899-1987
1098-2744
DOI:10.1002/mc.20401