Contribution of Fluorophores to Protein Kinase C FRET Probe Performance

Pairing probes: Recently, we prepared several probes that monitor protein kinase C activities in living cells based on a pleckstrin fragment sandwiched between two fluorescent proteins, GFP2 and EYFP. Herein, we replaced the fluorescent proteins (FPs) with monomeric variants which resulted in nonfun...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2008-06, Vol.9 (9), p.1379-1384
Main Authors: Jost, Christiane A, Reither, Gregor, Hoffmann, Carsten, Schultz, Carsten
Format: Article
Language:English
Subjects:
Amino Acid Sequence
bioorganic chemistry
Dimerization
Fluorescence Resonance Energy Transfer - methods
Fluorescence Resonance Energy Transfer - standards
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
imaging
kinases
microscopy
Microscopy, Fluorescence
Protein Kinase C - chemistry
Protein Kinase C - metabolism
Protein Structure, Quaternary
sensors
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Summary:Pairing probes: Recently, we prepared several probes that monitor protein kinase C activities in living cells based on a pleckstrin fragment sandwiched between two fluorescent proteins, GFP2 and EYFP. Herein, we replaced the fluorescent proteins (FPs) with monomeric variants which resulted in nonfunctional probes. This suggested that fluorophore dimerization actively participated in probe performance.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200700728