Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile

• Published in: Parasitology international 2008-09, Vol.57 (3), p.342-346
• Main Authors: Manterola, Carlos, Benavente, Felipe, Melo, Angélica, Vial, Manuel, Roa, Juan Carlos
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Chile - epidemiology; cox1 [MeSH]; Cyclooxygenase 1 - chemistry; Cyclooxygenase 1 - genetics; DNA, Helminth - analysis; DNA, Helminth - isolation & purification; Echinococcosis - epidemiology; Echinococcosis - parasitology; Echinococcus granulosus; Echinococcus granulosus - classification; Echinococcus granulosus - genetics; Echinococcus granulosus [MeSH]; Gastroenterology and Hepatology; Genotype; Genotypes [MeSH]; Humans; Infectious Disease; Molecular Sequence Data; PCR-RAPD; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2008.02.005

Abstract Introduction Echinococcus granulosus species has a wide variety in both geography and hosts; indeed, 10 genotypes have been reported in studies on material of animal origin. The aim of this study was to genotype E. granulosus obtained from human hydatid cysts. Materials and methods The hydatid fluid and sand was collected from patients who underwent surgery for hepatic and pulmonary hydatidosis at Hospital Regional in Temuco, Chile, between 2004 and 2005. Two PCR systems were used: PCR Eg9 and PCR Eg16. The RsaI enzyme was used for RFLP. The genotype was confirmed using the sequence of one fragment of 366 bp from a mitochondrial gene ( cox1 ). Results The DNA of protoscolices from 24 samples was analyzed, 4 of them from pulmonary cysts and 20 from hepatic cysts. The 366 bp fragment was amplified in 20 out of 24 samples (83.3%). Enzymatic digestion revealed the presence of 3 possible genotypes: in 20 out of 21 samples (95,2%), a restriction was observed corresponding to the G1 or G7 genotypes; in the remaining sample genotype G4 or G7 was observed. Sequencing confirmed the presence of G1 genotype for 19 samples and G6 genotype for the remaining sample (G4 or G7 according to PCR-RFLP). Conclusion The PCR-RFLP technique enabled three possible genotypes present (G1 or G7, G4 or G7) to be established. Sequencing allowed us to decisively identify the G1 and G6 genotypes in our study group. Previous studies agree with the identification of the G1 genotype in our country. We consider it significant that the G6 genotype is present in Chile for its epidemiological implications.