Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in Algeria

• Published in: Journal of antimicrobial chemotherapy 2008-07, Vol.62 (1), p.133-136
• Main Authors: Iabadene, Hassen, Messai, Yamina, Ammari, Houria, Ramdani-Bouguessa, Nadjia, Lounes, Saliha, Bakour, Rabah, Arlet, Guillaume
• Format: Article
• Language: English
• Subjects: Algeria; Anti-Bacterial Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacteria; beta-Lactamases - genetics; beta-Lactams - pharmacology; Biological and medical sciences; Cross Infection - microbiology; CTX-M; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Bacterial - genetics; E. cloacae; Enterobacter cloacae; Enterobacter cloacae - drug effects; Enterobacter cloacae - genetics; Enterobacter cloacae - isolation & purification; Enterobacteriaceae Infections - microbiology; Epidemiology; Gene expression; Genes, Bacterial; Genetics; Hospitals; Humans; Medical sciences; Microbial Sensitivity Tests; Microbiology; Pharmacology. Drug treatments; Polymerase Chain Reaction; QnrB; QnrS; Quinolones - pharmacology; Sequence Analysis, DNA; SHV-12
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkn145

Objectives The aim of this study is to evaluate the prevalence and diversity of extended-spectrum β-lactamases (ESBLs) in Enterobacter cloacae clinical isolates collected from Algerian hospitals and to verify the association with qnr genes. Methods MICs were determined by Etest for isolates giving positive double-disc synergy tests, and all isolates were screened by PCR and sequenced, respectively, for blaTEM, blaCTX-M, blaSHV and blaVEB genes and for qnr genes (qnrA, qnrB, qnrS), using specific primers. Results The prevalence of ESBLs was 25/141 (17.7%) with 11, 9, 4 and 1 isolates testing positive for genes encoding CTX-M-15, CTX-M-3, SHV-12 and VEB-1, respectively. Two SHV-12 producers and one CTX-M-15 producer expressed QnrS1, one isolate produced CTX-M-15 and QnrB1 and one SHV-12 producer co-expressed QnrS1 and QnrB4. qnrA was not detected in our collection, and qnr alleles were not detected in non-ESBL-producing isolates. Conclusions SHV-12, QnrS1, QnrB1 and QnrB4 were reported for the first time in Algeria. This study also described a co-expression of qnrS1 and qnrB4 by an SHV-12 producer isolate.