A Transient, Neuron-Wide Form of CREB-Mediated Long-Term Facilitation Can Be Stabilized at Specific Synapses by Local Protein Synthesis

In a culture system where a bifurcated Aplysia sensory neuron makes synapses with two motor neurons, repeated application of serotonin (5-HT) to one synapse produces a CREB-mediated, synapse-specific, long-term facilitation, which can be captured at the opposite synapse by a single pulse of 5-HT. Re...

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Bibliographic Details
Published in:Cell 1999-10, Vol.99 (2), p.221-237
Main Authors: Casadio, Andrea, Martin, Kelsey C, Giustetto, Maurizio, Zhu, Huixiang, Chen, Mary, Bartsch, Dusan, Bailey, Craig H, Kandel, Eric R
Format: Article
Language:English
Subjects:
Animals
Aplysia
Aplysia - physiology
Cells, Cultured
Cyclic AMP Response Element-Binding Protein - metabolism
Dactinomycin - pharmacology
Excitatory Postsynaptic Potentials - drug effects
Excitatory Postsynaptic Potentials - physiology
Gene Expression Regulation - drug effects
Long-Term Potentiation - drug effects
Long-Term Potentiation - physiology
Memory - physiology
Motor Neurons - drug effects
Motor Neurons - physiology
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Neurons, Afferent - drug effects
Neurons, Afferent - physiology
Protein Biosynthesis
Serotonin - pharmacology
Serotonin - physiology
Sirolimus - pharmacology
Synapses - drug effects
Synapses - physiology
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Summary:In a culture system where a bifurcated Aplysia sensory neuron makes synapses with two motor neurons, repeated application of serotonin (5-HT) to one synapse produces a CREB-mediated, synapse-specific, long-term facilitation, which can be captured at the opposite synapse by a single pulse of 5-HT. Repeated pulses of 5-HT applied to the cell body of the sensory neuron produce a CREB-dependent, cell-wide facilitation, which, unlike synapse-specific facilitation, is not associated with growth and does not persist beyond 48 hr. Persistent facilitation and synapse-specific growth can be induced by a single pulse of 5-HT applied to a peripheral synapse. Thus, the short-term process initiated by a single pulse of 5-HT serves not only to produce transient facilitation, but also to mark and stabilize any synapse of the neuron for long-term facilitation by means of a covalent mark and rapamycin-sensitive local protein synthesis.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)81653-0