Active human hepatitis B viral polymerase expressed in rabbit reticulocyte lysate system

Human HBV polymerase has been expressed in reticulocyte lysate system. The expressed protein shows the DNA-dependent DNA polymerase activity. In vitro transcription and translation produces a major protein product with an apparent molecular weight of approximately 100 kD. The HBV DNA polymerase has...

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Bibliographic Details
Published in:Virus genes 1999, Vol.19 (2), p.123-130
Main Authors: Kim, Y, Jung, G
Format: Article
Language:English
Subjects:
Animals
DNA-Directed DNA Polymerase - genetics
DNA-Directed DNA Polymerase - metabolism
Gene Products, pol - genetics
Gene Products, pol - metabolism
Hepatitis B
Hepatitis B virus
Hepatitis B virus - enzymology
magnesium chloride
manganese chloride
P protein
potassium chloride
Protein Biosynthesis
Rabbits
Reticulocytes
RNA-Directed DNA Polymerase - genetics
RNA-Directed DNA Polymerase - metabolism
sodium chloride
Transcription, Genetic
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Summary:Human HBV polymerase has been expressed in reticulocyte lysate system. The expressed protein shows the DNA-dependent DNA polymerase activity. In vitro transcription and translation produces a major protein product with an apparent molecular weight of approximately 100 kD. The HBV DNA polymerase has been characterized biochemically in the condition that the contaminating cellular DNA polymerases were fairly suppressed by aphidicolin and NEM. The polymerization reaction is optimal at pH 7.5 and 37 degrees C and the polymerase requires either MnCl2 or MgCl2, with a preference for MnCl2. The protein represented an optimal activity in the presence of either 75 mM NaCl or 100 mM KCl, with a higher activity at 75 mM NaCl than 100 mM KCl. Study of the polymerizing activity of the deleted versions of the polymerase protein suggests that the terminal protein is essential for full polymerase function and the spacer region may decrease the stability of the P protein.
ISSN:0920-8569
1572-994X
DOI:10.1023/A:1008175107309