Loading…

Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii

An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading f...

Full description

Saved in:
Bibliographic Details
Published in:Current genetics 1999-10, Vol.36 (4), p.215-221
Main Authors: Boretsky, Y, Voronovsky, A, Liuta-Tehlivets, O, Hasslacher, M, Kohlwein, S.D, Shavlovsky, G.M
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3
cites
container_end_page 221
container_issue 4
container_start_page 215
container_title Current genetics
container_volume 36
creator Boretsky, Y
Voronovsky, A
Liuta-Tehlivets, O
Hasslacher, M
Kohlwein, S.D
Shavlovsky, G.M
description An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.
doi_str_mv 10.1007/s002940050493
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69221028</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18323160</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3</originalsourceid><addsrcrecordid>eNqFkb1vFDEQxS1ERI5ASQuu6JaMP_Zsl1EUIFIkooTUK689vjPaXR_2LtL99_jYFFBRzbyZ37xiHiHvGHxiAOqyAHAjAVqQRrwgGyYFb8Bo8ZJsgCneaNDinLwu5QcA49qoV-ScQSuZbs2GLLcepzmG6Owc00RToHaiVw-PFAcc66pKTz3-wiEd_ugTQfdxt6cY6lnEyR3pnO1UQsrj6lKOZcaR1gG9j24fLd0tcRgi5jFNPsY35CzYoeDb53pBnj7ffL_-2tx9-3J7fXXXOCHl3ITgDddOcwOOOdV6HVqtFArLlFBc9SC9QtZvUUnmQ997g6x-Q_VKogpWXJCPq-8hp58LlrkbY3E4DHbCtJRuazhnwPV_QaYFF2wLFWxW0OVUSsbQHXIcbT52DLpTIN0_gVT-_bPx0o_o_6LXBCrwYQWCTZ3d5Vi6p0cOTJxcpKjNb7z3kDs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18323160</pqid></control><display><type>article</type><title>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</title><source>Springer Link</source><creator>Boretsky, Y ; Voronovsky, A ; Liuta-Tehlivets, O ; Hasslacher, M ; Kohlwein, S.D ; Shavlovsky, G.M</creator><creatorcontrib>Boretsky, Y ; Voronovsky, A ; Liuta-Tehlivets, O ; Hasslacher, M ; Kohlwein, S.D ; Shavlovsky, G.M</creatorcontrib><description>An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/s002940050493</identifier><identifier>PMID: 10541859</identifier><language>eng</language><publisher>United States</publisher><subject>3' Untranslated Regions ; Amino Acid Sequence ; amino acid sequences ; autonomous replication ; autonomously replicating sequence ; Base Sequence ; Blotting, Southern ; Cloning, Molecular ; DNA replication ; genbank/z49093 ; genes ; Genes, Fungal ; genetic transformation ; genetic vectors ; GTP Cyclohydrolase - genetics ; gtp cyclohydrolase ii ; hydrolases ; mitosis ; Molecular Sequence Data ; molecular seuqence data ; nucleotide sequences ; open reading frames ; Pichia ; Pichia - enzymology ; Pichia - genetics ; Pichia guilliermondii ; plasmid vectors ; Plasmids ; Replication Origin ; rib 1 gene ; RIB1 gene ; shuttle vectors ; Transformation, Genetic</subject><ispartof>Current genetics, 1999-10, Vol.36 (4), p.215-221</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10541859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boretsky, Y</creatorcontrib><creatorcontrib>Voronovsky, A</creatorcontrib><creatorcontrib>Liuta-Tehlivets, O</creatorcontrib><creatorcontrib>Hasslacher, M</creatorcontrib><creatorcontrib>Kohlwein, S.D</creatorcontrib><creatorcontrib>Shavlovsky, G.M</creatorcontrib><title>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</description><subject>3' Untranslated Regions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>autonomous replication</subject><subject>autonomously replicating sequence</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>DNA replication</subject><subject>genbank/z49093</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>genetic transformation</subject><subject>genetic vectors</subject><subject>GTP Cyclohydrolase - genetics</subject><subject>gtp cyclohydrolase ii</subject><subject>hydrolases</subject><subject>mitosis</subject><subject>Molecular Sequence Data</subject><subject>molecular seuqence data</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>Pichia</subject><subject>Pichia - enzymology</subject><subject>Pichia - genetics</subject><subject>Pichia guilliermondii</subject><subject>plasmid vectors</subject><subject>Plasmids</subject><subject>Replication Origin</subject><subject>rib 1 gene</subject><subject>RIB1 gene</subject><subject>shuttle vectors</subject><subject>Transformation, Genetic</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkb1vFDEQxS1ERI5ASQuu6JaMP_Zsl1EUIFIkooTUK689vjPaXR_2LtL99_jYFFBRzbyZ37xiHiHvGHxiAOqyAHAjAVqQRrwgGyYFb8Bo8ZJsgCneaNDinLwu5QcA49qoV-ScQSuZbs2GLLcepzmG6Owc00RToHaiVw-PFAcc66pKTz3-wiEd_ugTQfdxt6cY6lnEyR3pnO1UQsrj6lKOZcaR1gG9j24fLd0tcRgi5jFNPsY35CzYoeDb53pBnj7ffL_-2tx9-3J7fXXXOCHl3ITgDddOcwOOOdV6HVqtFArLlFBc9SC9QtZvUUnmQ997g6x-Q_VKogpWXJCPq-8hp58LlrkbY3E4DHbCtJRuazhnwPV_QaYFF2wLFWxW0OVUSsbQHXIcbT52DLpTIN0_gVT-_bPx0o_o_6LXBCrwYQWCTZ3d5Vi6p0cOTJxcpKjNb7z3kDs</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Boretsky, Y</creator><creator>Voronovsky, A</creator><creator>Liuta-Tehlivets, O</creator><creator>Hasslacher, M</creator><creator>Kohlwein, S.D</creator><creator>Shavlovsky, G.M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</title><author>Boretsky, Y ; Voronovsky, A ; Liuta-Tehlivets, O ; Hasslacher, M ; Kohlwein, S.D ; Shavlovsky, G.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>3' Untranslated Regions</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>autonomous replication</topic><topic>autonomously replicating sequence</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>DNA replication</topic><topic>genbank/z49093</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>genetic transformation</topic><topic>genetic vectors</topic><topic>GTP Cyclohydrolase - genetics</topic><topic>gtp cyclohydrolase ii</topic><topic>hydrolases</topic><topic>mitosis</topic><topic>Molecular Sequence Data</topic><topic>molecular seuqence data</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>Pichia</topic><topic>Pichia - enzymology</topic><topic>Pichia - genetics</topic><topic>Pichia guilliermondii</topic><topic>plasmid vectors</topic><topic>Plasmids</topic><topic>Replication Origin</topic><topic>rib 1 gene</topic><topic>RIB1 gene</topic><topic>shuttle vectors</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boretsky, Y</creatorcontrib><creatorcontrib>Voronovsky, A</creatorcontrib><creatorcontrib>Liuta-Tehlivets, O</creatorcontrib><creatorcontrib>Hasslacher, M</creatorcontrib><creatorcontrib>Kohlwein, S.D</creatorcontrib><creatorcontrib>Shavlovsky, G.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boretsky, Y</au><au>Voronovsky, A</au><au>Liuta-Tehlivets, O</au><au>Hasslacher, M</au><au>Kohlwein, S.D</au><au>Shavlovsky, G.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>36</volume><issue>4</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</abstract><cop>United States</cop><pmid>10541859</pmid><doi>10.1007/s002940050493</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0172-8083
ispartof Current genetics, 1999-10, Vol.36 (4), p.215-221
issn 0172-8083
1432-0983
language eng
recordid cdi_proquest_miscellaneous_69221028
source Springer Link
subjects 3' Untranslated Regions
Amino Acid Sequence
amino acid sequences
autonomous replication
autonomously replicating sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA replication
genbank/z49093
genes
Genes, Fungal
genetic transformation
genetic vectors
GTP Cyclohydrolase - genetics
gtp cyclohydrolase ii
hydrolases
mitosis
Molecular Sequence Data
molecular seuqence data
nucleotide sequences
open reading frames
Pichia
Pichia - enzymology
Pichia - genetics
Pichia guilliermondii
plasmid vectors
Plasmids
Replication Origin
rib 1 gene
RIB1 gene
shuttle vectors
Transformation, Genetic
title Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T14%3A32%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20an%20ARS%20element%20and%20development%20of%20a%20high%20efficiency%20transformation%20system%20for%20Pichia%20guilliermondii&rft.jtitle=Current%20genetics&rft.au=Boretsky,%20Y&rft.date=1999-10-01&rft.volume=36&rft.issue=4&rft.spage=215&rft.epage=221&rft.pages=215-221&rft.issn=0172-8083&rft.eissn=1432-0983&rft_id=info:doi/10.1007/s002940050493&rft_dat=%3Cproquest_cross%3E18323160%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=18323160&rft_id=info:pmid/10541859&rfr_iscdi=true