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Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii
An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading f...
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Published in: | Current genetics 1999-10, Vol.36 (4), p.215-221 |
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container_title | Current genetics |
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creator | Boretsky, Y Voronovsky, A Liuta-Tehlivets, O Hasslacher, M Kohlwein, S.D Shavlovsky, G.M |
description | An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed. |
doi_str_mv | 10.1007/s002940050493 |
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Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/s002940050493</identifier><identifier>PMID: 10541859</identifier><language>eng</language><publisher>United States</publisher><subject>3' Untranslated Regions ; Amino Acid Sequence ; amino acid sequences ; autonomous replication ; autonomously replicating sequence ; Base Sequence ; Blotting, Southern ; Cloning, Molecular ; DNA replication ; genbank/z49093 ; genes ; Genes, Fungal ; genetic transformation ; genetic vectors ; GTP Cyclohydrolase - genetics ; gtp cyclohydrolase ii ; hydrolases ; mitosis ; Molecular Sequence Data ; molecular seuqence data ; nucleotide sequences ; open reading frames ; Pichia ; Pichia - enzymology ; Pichia - genetics ; Pichia guilliermondii ; plasmid vectors ; Plasmids ; Replication Origin ; rib 1 gene ; RIB1 gene ; shuttle vectors ; Transformation, Genetic</subject><ispartof>Current genetics, 1999-10, Vol.36 (4), p.215-221</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10541859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boretsky, Y</creatorcontrib><creatorcontrib>Voronovsky, A</creatorcontrib><creatorcontrib>Liuta-Tehlivets, O</creatorcontrib><creatorcontrib>Hasslacher, M</creatorcontrib><creatorcontrib>Kohlwein, S.D</creatorcontrib><creatorcontrib>Shavlovsky, G.M</creatorcontrib><title>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</description><subject>3' Untranslated Regions</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>autonomous replication</subject><subject>autonomously replicating sequence</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>DNA replication</subject><subject>genbank/z49093</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>genetic transformation</subject><subject>genetic vectors</subject><subject>GTP Cyclohydrolase - genetics</subject><subject>gtp cyclohydrolase ii</subject><subject>hydrolases</subject><subject>mitosis</subject><subject>Molecular Sequence Data</subject><subject>molecular seuqence data</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>Pichia</subject><subject>Pichia - enzymology</subject><subject>Pichia - genetics</subject><subject>Pichia guilliermondii</subject><subject>plasmid vectors</subject><subject>Plasmids</subject><subject>Replication Origin</subject><subject>rib 1 gene</subject><subject>RIB1 gene</subject><subject>shuttle vectors</subject><subject>Transformation, Genetic</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkb1vFDEQxS1ERI5ASQuu6JaMP_Zsl1EUIFIkooTUK689vjPaXR_2LtL99_jYFFBRzbyZ37xiHiHvGHxiAOqyAHAjAVqQRrwgGyYFb8Bo8ZJsgCneaNDinLwu5QcA49qoV-ScQSuZbs2GLLcepzmG6Owc00RToHaiVw-PFAcc66pKTz3-wiEd_ugTQfdxt6cY6lnEyR3pnO1UQsrj6lKOZcaR1gG9j24fLd0tcRgi5jFNPsY35CzYoeDb53pBnj7ffL_-2tx9-3J7fXXXOCHl3ITgDddOcwOOOdV6HVqtFArLlFBc9SC9QtZvUUnmQ997g6x-Q_VKogpWXJCPq-8hp58LlrkbY3E4DHbCtJRuazhnwPV_QaYFF2wLFWxW0OVUSsbQHXIcbT52DLpTIN0_gVT-_bPx0o_o_6LXBCrwYQWCTZ3d5Vi6p0cOTJxcpKjNb7z3kDs</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Boretsky, Y</creator><creator>Voronovsky, A</creator><creator>Liuta-Tehlivets, O</creator><creator>Hasslacher, M</creator><creator>Kohlwein, S.D</creator><creator>Shavlovsky, G.M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19991001</creationdate><title>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</title><author>Boretsky, Y ; Voronovsky, A ; Liuta-Tehlivets, O ; Hasslacher, M ; Kohlwein, S.D ; Shavlovsky, G.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-ffd928c8290c1c75d8f5877e3a173727b04d7e1b6e741dfbbd9e12947b74e7fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>3' Untranslated Regions</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>autonomous replication</topic><topic>autonomously replicating sequence</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>DNA replication</topic><topic>genbank/z49093</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>genetic transformation</topic><topic>genetic vectors</topic><topic>GTP Cyclohydrolase - genetics</topic><topic>gtp cyclohydrolase ii</topic><topic>hydrolases</topic><topic>mitosis</topic><topic>Molecular Sequence Data</topic><topic>molecular seuqence data</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>Pichia</topic><topic>Pichia - enzymology</topic><topic>Pichia - genetics</topic><topic>Pichia guilliermondii</topic><topic>plasmid vectors</topic><topic>Plasmids</topic><topic>Replication Origin</topic><topic>rib 1 gene</topic><topic>RIB1 gene</topic><topic>shuttle vectors</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boretsky, Y</creatorcontrib><creatorcontrib>Voronovsky, A</creatorcontrib><creatorcontrib>Liuta-Tehlivets, O</creatorcontrib><creatorcontrib>Hasslacher, M</creatorcontrib><creatorcontrib>Kohlwein, S.D</creatorcontrib><creatorcontrib>Shavlovsky, G.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boretsky, Y</au><au>Voronovsky, A</au><au>Liuta-Tehlivets, O</au><au>Hasslacher, M</au><au>Kohlwein, S.D</au><au>Shavlovsky, G.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>36</volume><issue>4</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>An Autonomously Replicating Sequence element adjacent to the RIB1 gene encoding GTP cyclohydrolase II of the yeast Pichia guilliermondii was identified by transformation experiments. Detailed sequence analysis unveiled two potential ARS elements located 5' and 3' of the RIB1 open reading frame. The chromosomal fragment containing the ARS-like sequence 3' to the RIB1 structural gene, called PgARS, conferred high transformation frequencies of 10(4)-10(5) transformants/microg of DNA to a pUC19-derived plasmid in P. guilliermondii. The PgARS element also conferred autonomous replication to hybrid plasmids in this host. Based on this element a series of Escherichia coli shuttle vectors for efficient transformation of the flavinogenic yeast P. guilliermondii was developed.</abstract><cop>United States</cop><pmid>10541859</pmid><doi>10.1007/s002940050493</doi><tpages>7</tpages></addata></record> |
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subjects | 3' Untranslated Regions Amino Acid Sequence amino acid sequences autonomous replication autonomously replicating sequence Base Sequence Blotting, Southern Cloning, Molecular DNA replication genbank/z49093 genes Genes, Fungal genetic transformation genetic vectors GTP Cyclohydrolase - genetics gtp cyclohydrolase ii hydrolases mitosis Molecular Sequence Data molecular seuqence data nucleotide sequences open reading frames Pichia Pichia - enzymology Pichia - genetics Pichia guilliermondii plasmid vectors Plasmids Replication Origin rib 1 gene RIB1 gene shuttle vectors Transformation, Genetic |
title | Identification of an ARS element and development of a high efficiency transformation system for Pichia guilliermondii |
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