Protection of Mice against Challenge with Foot and Mouth Disease Virus (FMDV) by Immunization with Foliar Extracts from Plants Infected with Recombinant Tobacco Mosaic Virus Expressing the FMDV Structural Protein VP1

A tobacco mosaic virus (TMV)-based vector has been used to express in plants the complete open reading frame coding for VP1, the major immunogenic protein of foot and mouth disease virus (FMDV). In vitro RNA transcripts were inoculated into Nicotiana benthamiana plants and detectable amounts of reco...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1999-11, Vol.264 (1), p.85-91
Main Authors: Wigdorovitz, A., Pérez Filgueira, D.M., Robertson, N., Carrillo, C., Sadir, A.M., Morris, T.J., Borca, M.V.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Antibody Formation
Aphthovirus - genetics
Aphthovirus - immunology
Capsid - genetics
Capsid - immunology
Capsid Proteins
Enzyme-Linked Immunosorbent Assay
FMDV
Foot-and-Mouth Disease - immunology
Foot-and-Mouth Disease - prevention & control
foot-and-mouth disease virus
genetic vectors
infectious immunity—virus
leaves
Mice
Nicotiana benthamiana
Plants - virology
recombinant vaccines
Suid herpesvirus 1
TMV
Tobacco mosaic virus
Tobacco Mosaic Virus - genetics
vaccination
Vaccines, Synthetic
Viral Vaccines
viral vectors
VP1 protein
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Description
Summary:A tobacco mosaic virus (TMV)-based vector has been used to express in plants the complete open reading frame coding for VP1, the major immunogenic protein of foot and mouth disease virus (FMDV). In vitro RNA transcripts were inoculated into Nicotiana benthamiana plants and detectable amounts of recombinant VP1 were identified by Western blot as soon as 4 days postinfection. Foliar extracts prepared from infected leaves were injected intraperitoneally into mice and all of the immunized animals developed a specific antibody response to both the complete virus particle and the major immunogenic region as determined by ELISA and Western blot analysis. Most importantly, all immunized mice developed a protective immune response against experimental challenge with virulent FMDV. To our knowledge, this is the first report showing the expression of a complete open reading frame of an antigenic foreign protein in plants, using a recombinant plant virus, in sufficient quantity to permit use of the crude plant extract as an experimental immunogen to protect animals against virus challenge.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1999.9923