Construction of Epstein–Barr Virus-Based Expression Vector Containing Mini-OriP

Epstein–Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effe...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-11, Vol.264 (3), p.938-943
Main Authors: Tanaka, Junko, Miwa, Yoshihiro, Miyoshi, Keiko, Ueno, Akemichi, Inoue, Hideo
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Epidermal Growth Factor - genetics
Epstein-Barr virus
Gene Transfer Techniques
Genes, Reporter
Genetic Engineering
Genetic Therapy
Genetic Vectors
Herpesvirus 4, Human
Humans
Mice
Rats
Replication Origin - genetics
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Summary:Epstein–Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1617