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Effects of the cFMS Kinase Inhibitor 5-(3-Methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (GW2580) in Normal and Arthritic Rats
The cFMS (cellular homolog of the V-FMS oncogene product of the Susan McDonough strain of feline sarcoma virus) ( Proc Natl Acad Sci U S A 83: 3331â3335, 1986) kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (GW2580) inhibits colony-stimulating factor (CSF)-1-in...
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Published in: | The Journal of pharmacology and experimental therapeutics 2008-07, Vol.326 (1), p.41-50 |
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Main Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The cFMS (cellular homolog of the V-FMS oncogene product of the Susan McDonough strain of feline sarcoma virus) ( Proc Natl Acad Sci U S A 83: 3331â3335, 1986) kinase inhibitor 5-(3-methoxy-4-((4-methoxybenzyl)oxy)benzyl)pyrimidine-2,4-diamine (GW2580) inhibits colony-stimulating
factor (CSF)-1-induced monocyte growth and bone degradation in vitro and inhibits CSF-1 signaling through cFMS kinase in 4-day
models in mice ( Proc Natl Acad Sci U S A 102: 16078, 2005). In the present study, the kinase selectivity of GW2580 was further characterized, and the effects of chronic
treatment were evaluated in normal and arthritic rats. GW2580 selectively inhibited cFMS kinase compared with 186 other kinases
in vitro and completely inhibited CSF-1-induced growth of rat monocytes, with an IC 50 value of 0.2 μM. GW2580 dosed orally at 25 and 75 mg/kg 1 and 5 h before the injection of lipopolysaccharide inhibited tumor
necrosis factor-α production by 60 to 85%, indicating a duration of action of at least 5 h. In a 21-day adjuvant arthritis
model, GW2580 dosed twice a day (b.i.d.) from days 0 to 21, 7 to 21, or 14 to 21 inhibited joint connective tissue and bone
destruction as assessed by radiology, histology and bone mineral content measurements. In contrast, GW2580 did not affect
ankle swelling in the adjuvant model nor did it affect ankle swelling in a model where local arthritis is reactivated by peptidoglycan
polysaccharide polymers. GW2580 administered to normal rats for 21 days showed no effects on tissue histology and only modest
changes in serum clinical chemistry and blood hematology. In conclusion, GW2580 was effective in preserving joint integrity
in the adjuvant arthritis model while showing minimal effects in normal rats. |
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ISSN: | 0022-3565 1521-0103 |
DOI: | 10.1124/jpet.107.129429 |