Genetic analysis of phenylacetic acid catabolism in Arthrobacter oxydans CECT386

Arthrobacter oxydans CECT386 is a Gram-positive bacterium able to use either phenylacetic acid or phenylacetaldehyde as the sole carbon and energy source for aerobic growth. Genes responsible for the catabolism of these compounds have been located at two chromosomal regions and were organized in one...

Full description

Saved in:
Bibliographic Details
Published in:Archives of microbiology 2008-07, Vol.190 (1), p.89-100
Main Authors: Navarro-Llorens, Juana María, Drzyzga, Oliver, Perera, Julián
Format: Article
Language:English
Subjects:
Acids
Aromatic compounds
Arthrobacter
Arthrobacter - genetics
Arthrobacter - metabolism
Bacteria
Bacteriology
Biochemistry
biodegradation
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbon
Cell Biology
Chromosomes, Bacterial - genetics
Cloning, Molecular
DNA, Bacterial - genetics
E coli
Ecology
Energy sources
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetic Complementation Test
Glycerol
Gram-negative bacteria
Gram-positive bacteria
Life Sciences
Metabolism
Metabolites
Microbial Ecology
Microbiology
Miscellaneous
Molecular Sequence Data
Multigene Family
Open Reading Frames
Operon
Original Paper
Phenols
Phenylacetates - metabolism
phenylacetic acid
Plasmids
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Arthrobacter oxydans CECT386 is a Gram-positive bacterium able to use either phenylacetic acid or phenylacetaldehyde as the sole carbon and energy source for aerobic growth. Genes responsible for the catabolism of these compounds have been located at two chromosomal regions and were organized in one isolated paaN gene and two putative paa operons, one consisting of the paaD, paaF, tetR and prot genes, and one consisting of the paaG, paaH, paaI, paaJ, paaK and paaB genes. The identity of the paaF and paaN genes was supported by functional complementation experiments. A comparison with the paa catabolic genes and/or gene clusters of other bacteria that degrade these aromatic compounds is presented. The results of this study broaden the knowledge regarding the range of metabolic potential of this strain and eventually make it attractive for environmental applications.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-008-0370-x