Apoptosis Is Induced by Hantaviruses in Cultured Cells

Hantaviruses replicate in primary and cultured animal cells with little or no cytopathic effect. We report here that the cultured Vero E6 cells infected by the Hantaan or by the Prospect Hill viruses exhibited characteristic features of apoptosis, including condensation and segmentation of nuclei an...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1999-11, Vol.264 (1), p.99-105
Main Authors: Kang, Ju-Il, Park, Se-Hoon, Lee, Pyung-Woo, Ahn, Byung-Yoon
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Cell Nucleus - ultrastructure
Cell Nucleus - virology
Cell Survival
Cercopithecus aethiops
DNA Fragmentation
Hantaan virus
Hantaan virus - physiology
Hantaan virus - radiation effects
Hantavirus
Hantavirus - physiology
Hantavirus - radiation effects
In Situ Nick-End Labeling
Nucleosomes - ultrastructure
Nucleosomes - virology
Prospect hill virus
Ultraviolet Rays
Vaccinia virus - physiology
Vero Cells
Virus Replication
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hantaviruses replicate in primary and cultured animal cells with little or no cytopathic effect. We report here that the cultured Vero E6 cells infected by the Hantaan or by the Prospect Hill viruses exhibited characteristic features of apoptosis, including condensation and segmentation of nuclei and internucleosomal cleavage of nuclear DNA. Apoptosis was not seen in the cells adsorbed by UV-inactivated virus, indicating that the viral replication is required for the induction of apoptosis. Furthermore, level of the proto-oncogenic Bcl-2 protein was significantly reduced, whereas its mRNA level remained unchanged in Hantaan virus-infected cells, suggesting possible involvement and posttranscriptional regulation of this antiapoptotic protein in the process.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1999.9896