Genetic contribution of the CD14 -159C/T dimorphism in the promoter region in Japanese RA

• Published in: Clinical and experimental rheumatology 2008-03, Vol.26 (2), p.337-339
• Main Authors: TAKEUCHI, F, NAKAUE, N, KOBAYASHI, N, KUWATA, S, MURAYAMA, T, KAWASUGI, K, MORI, M, MATSUTA, K
• Format: Article
• Language: English
• Subjects: Aged; Arthritis, Rheumatoid - ethnology; Arthritis, Rheumatoid - genetics; Biological and medical sciences; Diseases of the osteoarticular system; Female; Genetic Predisposition to Disease - ethnology; Genotype; Humans; Inflammatory joint diseases; Japan - epidemiology; Lipopolysaccharide Receptors - genetics; Male; Medical sciences; Middle Aged; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Prevalence; Promoter Regions, Genetic - genetics
• ISSN: 0392-856X; 1593-098X

To study the contribution of the CD14 gene to the pathogenesis of rheumatoid arthritis (RA) in Japanese patients. CD14 genotyping was carried out at the -159C/T dimorphic site in 97 RA patients and 104 normal subjects by the PCR-RFLP (restriction fragment length polymorphism) HLA-DRB1 genotyping was performed by the PCR-SSCP (sequence specific conformational polymorphism) method. The -159C/T dimorphism is not associated with whole RA or with female RA, and the results were compatible with a previous report from Germany. The -159C/T dimorphism was not associated with rheumatoid factor (RF)-positive RA, although the -159T allele tended to be associated with RF in the German report. The -159C/T dimorphism showed no association even in RA patients with the RA-susceptibility HLA-DRB1*0405. The -159T allele was prevalent in Japanese controls. The CD14 gene is very unlikely to be genetically involved in the pathogenesis of Japanese RA.