Constructing an in vitro Cornea from Cultures of the Three Specific Corneal Cell Types

This paper presents a reliable method for establishing pure cultures of the three types of corneal cells. This is believed to be the first time, corneal cells have been cultured from fetal pig corneas. Cell growth studies were performed in different media. Subcultures of the three corneal cell types...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 1999-10, Vol.35 (9), p.515-526
Main Authors: Schneider, Adelheid I, Maier-Reif, Katharina, Graeve, Thomas
Format: Article
Language:English
Subjects:
Animals
Basement membrane
Cell Culture Techniques
Cell Division - physiology
Cell growth
Cellular Models
Collagens
Cornea
Cornea - cytology
Cornea - growth & development
Corneal Stroma - cytology
Culture Media
Cultured cells
Endothelial cells
Endothelium, Corneal - cytology
Epithelial cells
Epithelium, Corneal - cytology
Fetus - cytology
Gels
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Stromal cells
Swine
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Summary:This paper presents a reliable method for establishing pure cultures of the three types of corneal cells. This is believed to be the first time, corneal cells have been cultured from fetal pig corneas. Cell growth studies were performed in different media. Subcultures of the three corneal cell types were passaged until the 30th generation without their showing signs of senescence. For engineering an in vitro cornea, corneal epithelial cells were cultured over corneal stromal cells in an artificial biomatrix of collagen with an underlying layer of corneal endothelial cells. The morphology, histology, and differentiation of the in vitro cornea were investigated to determine the degree of comparability to the cornea in vivo. The in vitro construct displayed signs of transition to an organotypic phenotype of which the most prominent was the formation of two basement membranes.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-999-0062-0