Protein carbonyl levels in serum and gingival crevicular fluid in patients with chronic periodontitis

Abstract Objective Evidence reveals the role of reactive oxygen species (ROS) in many pathologies including periodontitis. Protein carbonylation is the most widely used biomarker for oxidative damage to proteins, and reflects cellular damage induced by ROS. In this study protein carbonyl (PC) levels...

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Bibliographic Details
Published in:Archives of oral biology 2008-08, Vol.53 (8), p.716-722
Main Authors: Baltacıoğlu, Esra, Akalın, Ferda Alev, Alver, Ahmet, Değer, Orhan, Karabulut, Erdem
Format: Article
Language:English
Subjects:
Adult
Advanced Basic Science
Biomarkers - metabolism
Chronic Disease
Dentistry
Female
Gingival crevicular fluid
Gingival Crevicular Fluid - chemistry
Humans
Male
Middle Aged
Oxidative Stress
Periodontal Index
Periodontitis
Periodontitis - blood
Periodontitis - metabolism
Protein Carbonylation
Protein carbonyls
Protein oxidation
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Summary:Abstract Objective Evidence reveals the role of reactive oxygen species (ROS) in many pathologies including periodontitis. Protein carbonylation is the most widely used biomarker for oxidative damage to proteins, and reflects cellular damage induced by ROS. In this study protein carbonyl (PC) levels in serum and gingival crevicular fluid (GCF) in patients with chronic periodontitis (CP) was evaluated. Materials and methods Thirty-three patients with CP and 24 healthy controls were included in the study. Following the clinical measurements and samplings, total protein levels in serum and GCF were determined by Bradford method, and serum and GCF PC levels were measured by modified Levine method. Results PC levels in serum and GCF were significantly higher in the CP group compared to the control group ( p < 0.05). In all subjects, serum and GCF PC levels showed statistically significant positive correlations with all clinical parameters ( p < 0.05). Conclusions The results suggest that both systemic and local/periodontal protein carbonylation increase in CP compared to health and that elevated levels of PCs may be a sign of oxidative damage in periodontitis and correlate well with the periodontal status.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2008.02.002