The major acidic glycolipids from the kidney of the Pacific salmon (Oncorhynchus keta): characterization of a novel ganglioside, fucosyl-N-acetylgalactosaminyl-GM1

Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (X1) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methyla...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1999-11, Vol.126 (5), p.962-968
Main Authors: Niimura, Y, Tomori, M, Tadano-Aritomi, K, Toida, T, Ishizuka, I
Format: Article
Language:English
Subjects:
Animals
Carbohydrate Conformation
Carbohydrate Sequence
G(M1) Ganglioside - analogs & derivatives
G(M1) Ganglioside - chemistry
G(M1) Ganglioside - isolation & purification
Gangliosides - chemistry
Gangliosides - isolation & purification
Glycolipids - chemistry
Glycolipids - isolation & purification
Kidney - chemistry
Magnetic Resonance Spectroscopy
Mass Spectrometry
Molecular Sequence Data
Oncorhynchus keta - metabolism
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Summary:Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (X1) on TLC was separated from the slower moving one (X2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and (1)H-NMR spectroscopy, X1 was determined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique Fucalpha1-3GalNAc linkage at the nonreducing terminal: Fucalpha1-3GalNAcbeta1-3Galbeta1-3GalNAcbeta1-4[ NeuAcalpha2-3]Galbeta 1-4Glcbeta1-1Cer. Analysis of the lipophilic moiety indicated predominance of 24:1 fatty acid in combination with sphingenine. X2 was found to have a glycon structure identical to X1. The ceramide of X2 consisted predominantly of saturated fatty acids (18:0 and 16:0). The tissue concentrations of X1 and X2 in kidney were 3.7 and 2.8 nmol/g, respectively.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a022541