Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms

• Published in: Avian pathology 2008-06, Vol.37 (3), p.273-279
• Main Authors: Yu, C.Y, Liu, Y.W, Chou, S.J, Chao, M.R, Weng, B.C, Tsay, J.G, Chiu, C.H, Wu, C.C, Lin, T.L, Chang, C.C
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; bacterial infections; Base Sequence; Cluster analysis; disease outbreaks; Disease Outbreaks - veterinary; DNA, Bacterial - genetics; Drug resistance; ducks; Ducks - microbiology; farms; geese; Geese - microbiology; Genetic Variation; Genome, Bacterial; Genomics; genotype; Genotype & phenotype; Gram-Negative Bacteria - genetics; Gram-Negative Bacterial Infections - epidemiology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; infectious serositis; microbial genetics; molecular seqence data; multiple drug resistance; nucleotide sequences; pathogen identification; Pathology; Phylogeny; plasmid profiling; plasmids; polymerase chain reaction; Polymorphism; poultry diseases; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; pulsed-field restriction fragment length polymorphism; repetitive-sequence polymerase chain reaction; restriction fragment length polymorphism; Riemerella anatipestifer; serotypes; Wildfowl
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079450802056546

Riemerella anatipestifer causes infectious serositis of ducks and geese. The genomic diversity of R. anatipestifer associated with outbreaks in waterfowls was studied using 24 multidrug-resistant R. anatipestifer isolates collected from the visceral organs of ducks and geese from seven outbreaks in four goose farms and one outbreak in one duck farm. Seven methods were used to differentiate these isolates. Plasmid patterns differed in plasmid number and size, ranging from 2.9 kb to 20 kb, and provided seven profiles. Divergent nucleotide sequences (predominant in 670 to 830 base pairs) of the ompA gene categorized the 24 isolates into three groups based on cluster analysis and polymerase chain reaction-restriction fragment length polymorphism. Repetitive-sequence polymerase chain reaction and pulsed-field gel electrophoresis analysis revealed the highest genotypic variations among the isolates. Genotypes and serotypes differed among farms and within the same farm and even within a single goose. In conclusion, a difference in R. anatipestifer genotypes and serotypes was observed for multiple outbreaks in waterfowls.