Construction of an infectious full-length cDNA clone of potato virus M

An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflo...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2008-07, Vol.153 (7), p.1385-1389
Main Authors: Flatken, S, Ungewickell, V, Menzel, W, Maiss, E
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Agrobacterium tumefaciens - genetics
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brief Report
Carlavirus - genetics
Cauliflower mosaic virus
Cloning
Cloning, Molecular
DNA polymerase
DNA, Complementary - genetics
Electroporation
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gene Order
Genomes
Hesperis
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Nicotiana
Phytopathology. Animal pests. Plant and forest protection
Plant Diseases - virology
Plant viruses and viroids
Plants - virology
Plasmids
Potato virus M
Potatoes
Proteins
RNA, Viral - genetics
Virology
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-008-0127-5