Use of response surface optimization for the production of biosurfactant from Rhodococcus spp. MTCC 2574

The production of biosurfactant from Rhodococcus spp. MTCC 2574 was effectively enhanced by response surface methodology (RSM). Rhodococcus spp. MTCC 2574 was selected through screening of seven different Rhodococcus strains. The preliminary screening experiments (one-factor at a time) suggested tha...

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Bibliographic Details
Published in:Bioresource technology 2008-11, Vol.99 (16), p.7875-7880
Main Authors: Mutalik, Snehal R., Vaidya, Bhalchandra K., Joshi, Renuka M., Desai, Kiran M., Nene, Sanjay N.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosurfactant
Biotechnology
Carbon - metabolism
Culture Media - chemistry
Fundamental and applied biological sciences. Psychology
Mannitol - metabolism
Medium optimization
Micelles
Nitrogen - metabolism
Response surface methodology
Rhodococcus
Rhodococcus - genetics
Rhodococcus - metabolism
Rhodococcus spp
Surface Properties
Surface Tension
Surface-Active Agents - metabolism
Water
Yeasts
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Summary:The production of biosurfactant from Rhodococcus spp. MTCC 2574 was effectively enhanced by response surface methodology (RSM). Rhodococcus spp. MTCC 2574 was selected through screening of seven different Rhodococcus strains. The preliminary screening experiments (one-factor at a time) suggested that carbon source: mannitol, nitrogen source: yeast extract and meat peptone and inducer: n-hexadecane are the critical medium components. The concentrations of these four media components were optimized by using central composite rotatable design (CCRD) of RSM. The adequately high R2 value (0.947) and F score 19.11 indicated the statistical significance of the model. The optimum medium composition for biosurfactant production was found to contain mannitol (1.6g/L), yeast extract (6.92g/L), meat peptone (19.65g/L), n-hexadecane (63.8g/L). The crude biosurfactant was obtained from methyl tert-butyl ether extraction. The yield of biosurfactant before and after optimization was 3.2g/L of and 10.9g/L, respectively. Thus, RSM has increased the yield of biosurfactant to 3.4-fold. The crude biosurfactant decreased the surface tension of water from 72mN/m to 30.8mN/m (at 120mgL−1) and achieved a critical micelle concentration (CMC) value of 120mgL−1.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2008.02.027