LOH at the APC/MCC gene (5Q21) is frequent in early stages of non-small cell lung cancer

Lung cancer is the leading cause of death in both women and men in the United States and many European countries. Molecular cytogenetic and LOH analyses of non-small cell lung cancer have shown somatic genetic alterations in a variety of chromosomes, such as 1p, 3p, 5q, 8p, 9p, 11p, 11q and 17p. All...

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Bibliographic Details
Published in:Pathology, research and practice research and practice, 1999-01, Vol.195 (10), p.677-680
Main Authors: Sanz-Ortega, J, Bryant, B, Sanz-Esponera, J, Asenjo, J A, Saez, M C, Torres, A, Balibrea, J L, Sobel, M E, Merino, M J
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - mortality
Adenomatous Polyposis Coli Protein
Adult
Aged
Aged, 80 and over
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - mortality
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - mortality
Chromosomes, Human, Pair 5 - genetics
Cytoskeletal Proteins - genetics
Female
Genetic Markers
Humans
Loss of Heterozygosity - genetics
Lung Neoplasms - genetics
Lung Neoplasms - mortality
Male
Middle Aged
Survival Rate
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Summary:Lung cancer is the leading cause of death in both women and men in the United States and many European countries. Molecular cytogenetic and LOH analyses of non-small cell lung cancer have shown somatic genetic alterations in a variety of chromosomes, such as 1p, 3p, 5q, 8p, 9p, 11p, 11q and 17p. Allelic deletions of the known tumor suppressor gene APC at 5q21 are frequently observed in advanced stages of lung cancer and have been correlated with poor prognosis in previous reports. We investigated 33 cases of NSCL for LOH at 5q21: 22 squamous cell and 11 adenocarcinomas. Normal and tumor cells were microdissected from paraffin embedded tissues and PCR amplification was performed utilising the specific markers D5S299 and D5S346 at 5q21 and PYGM at 11q13, respectively. Clinicopathological data, survival and recurrence rates were obtained in all cases. We detected LOH at 5q21 in 4/9 (44%) informative adenocarcinomas and in 13/16 (81%) informative SCC. LOH was frequent in early stages (12/15 stage I cases) and did not correlate with recurrence or poor survival. Our results show that LOH at 5q21 is more frequent in squamous cell carcinomas than in adenocarcinomas, is frequent in early stages of the disease, and does not have prognostic significance.
ISSN:0344-0338
DOI:10.1016/S0344-0338(99)80058-2