Upregulation of miR-23a approximately 27a approximately 24 decreases transforming growth factor-beta-induced tumor-suppressive activities in human hepatocellular carcinoma cells

Transforming growth factor-beta (TGF-beta) plays a dual and complex role in human cancer. In this report, we observe a specific set of MicroRNAs (miRNAs) changed in response to TGF-beta in human hepatocellular carcinoma (HCC) cells by miRNA microarray screening. A cluster of miRNA, miR-23a approxima...

Full description

Saved in:
Bibliographic Details
Published in:International journal of cancer 2008-08, Vol.123 (4), p.972-978
Main Authors: Huang, Shenglin, He, Xianghuo, Ding, Jie, Liang, Linhui, Zhao, Yingjun, Zhang, Zhenfeng, Yao, Xiao, Pan, Zhimei, Zhang, Pingping, Li, Jinjun, Wan, Dafang, Gu, Jianren
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Cell Growth Processes - physiology
Cell Line, Tumor
Humans
Lentivirus - genetics
Liver Neoplasms - drug therapy
Liver Neoplasms - genetics
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
MicroRNAs - biosynthesis
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis
Smad Proteins - metabolism
Transfection
Transforming Growth Factor beta - metabolism
Transforming Growth Factor beta - pharmacology
Up-Regulation - drug effects
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transforming growth factor-beta (TGF-beta) plays a dual and complex role in human cancer. In this report, we observe a specific set of MicroRNAs (miRNAs) changed in response to TGF-beta in human hepatocellular carcinoma (HCC) cells by miRNA microarray screening. A cluster of miRNA, miR-23a approximately 27a approximately 24, is induced in an early stage by TGF-beta in Huh-7 cells. Knockdown of Smad4, Smad2 or Smad3 expression by RNA interference can attenuate the response of miR-23a approximately 27a approximately 24 to TGF-beta addition, indicating that this induction is dependent on Smad pathway. We also explore that miR-23a approximately 27a approximately 24 can function as an antiapoptotic and proliferation-promoting factor in liver cancer cells. In addition, expression of this miRNA cluster is found to be remarkably upregulated in HCC tissues versus normal liver tissues. These findings suggest a novel, alternative mechanism through which TGF-beta could induce specific miRNA expression to escape from tumor-suppressive response in HCC cells.
ISSN:1097-0215
DOI:10.1002/ijc.23580