Ginsenoside Rh2 induces apoptosis independently of Bcl-2, Bcl-xL, or Bax in C6Bu-1 cells

In ginsenoside Rh2-treated rat glioma C6Bu-1 cells, apoptotic morphological changes, such as cell shrinkage, chromatin condensation and pyknosis were confirmed by means of electron microscopy. To evaluate whether induction of apoptosis by ginsenoside Rh2 is mediated by the members of Bcl-2 family, w...

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Bibliographic Details
Published in:Archives of pharmacal research 1999-10, Vol.22 (5), p.448-453
Main Authors: Kim, Y S, Jin, S H, Lee, Y H, Kim, S I, Park, J D
Format: Article
Language:English
Subjects:
Animals
Apoptosis
bcl-2-Associated X Protein
bcl-X Protein
Blotting, Western
Cell Survival - drug effects
Ginsenosides
Microscopy, Electron
Panax - chemistry
Plants, Medicinal
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats
Saponins - pharmacology
Time Factors
Tumor Cells, Cultured
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Summary:In ginsenoside Rh2-treated rat glioma C6Bu-1 cells, apoptotic morphological changes, such as cell shrinkage, chromatin condensation and pyknosis were confirmed by means of electron microscopy. To evaluate whether induction of apoptosis by ginsenoside Rh2 is mediated by the members of Bcl-2 family, we first established C6Bu-1 cells overexpressing Bcl-2. It was demonstrated that the expression of Bcl-2, Bcl-xL and Bax was not altered in ginsenoside Rh2-treated C6Bu-1 cells. Bcl-2 overexpressing C6Bu-1 cells failed to prevent from ginsenoside Rh2-induced cell death. These results suggest the existence of other apoptotic pathway that requires induction of apoptosis by ginsenoside Rh2 rather than the pathway through Bcl-2, Bcl-xL or Bax in C6Bu-1 cells.
ISSN:0253-6269
1976-3786
DOI:10.1007/BF02979151