Protoporphyrin IX fluorescence photobleaching is a useful tool to predict the response of rat ovarian cancer following hexaminolevulinate photodynamic therapy

Objective Accurate dosimetry was shown to be critical to achieve effective photodynamic therapy (PDT). This study aimed to assess the reliability of in vivo protoporphyrin IX (PpIX) fluorescence photobleaching as a predictive tool of the hexaminolevulinate PDT (HAL‐PDT) response in a rat model of ad...

Full description

Saved in:
Bibliographic Details
Published in:Lasers in surgery and medicine 2008-07, Vol.40 (5), p.332-341
Main Authors: Ascencio, Manuel, Collinet, Pierre, Farine, M.O., Mordon, Serge
Format: Article
Language:English
Subjects:
Adenocarcinoma - drug therapy
Adenocarcinoma - pathology
Aminolevulinic Acid - analogs & derivatives
Aminolevulinic Acid - therapeutic use
Animals
dosimetry
Female
fluorescence
hexaminolevulinate
Microscopy, Fluorescence
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - pathology
Photobleaching
Photochemotherapy
photodynamic therapy
Photosensitizing Agents - therapeutic use
Predictive Value of Tests
protoporphyrin IX
Protoporphyrins
Rats
Rats, Inbred F344
Reproducibility of Results
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective Accurate dosimetry was shown to be critical to achieve effective photodynamic therapy (PDT). This study aimed to assess the reliability of in vivo protoporphyrin IX (PpIX) fluorescence photobleaching as a predictive tool of the hexaminolevulinate PDT (HAL‐PDT) response in a rat model of advanced ovarian cancer. Materials and Methods Intraperitoneal 106 NuTu 19 cells were injected in 26 female rats Fisher 344. Peritoneal carcinomatosis was obtained 26 days post‐tumor induction. Four hours post‐intraperitoneal HAL (Photocure ASA, Oslo, Norway) injection, a laparoscopic procedure (D‐light AutoFluorescence system, Karl Storz endoscope, Tuttlingen, Germany) and a fluorescence examination were made for 22 rats. The first group (LASER group, n = 26) was illuminated with laser light using a 532 nm KTP laser (Laser Quantum, Stockport, UK) on 1 cm2 surface at 45 J/cm2. The second group (NO LASER group, n = 26) served as controls. Biopsies were taken 24 hours after PDT. Semi‐quantitative histology was performed and necrosis value was determined: 0—no necrosis to 4—full necrosis. Fluorescence was monitored before and after illumination on complete responders (NV = 3–4; n = 20) and non‐responders (NV = 0–2; n = 6). Results High PpIX photobleaching corresponded with complete responders whereas low photobleaching corresponded with non‐responders (P
ISSN:0196-8092
1096-9101
DOI:10.1002/lsm.20629