Characterization of human rabies virus vaccine strain in China

Human rabies virus vaccine strain CTN181 from China was sequenced. The overall length of the genome was 11,923 nucleotides (nt), comprising a leader sequence of 58nt, nucleoprotein (N) gene of 1353nt, phosphoprotein (P) gene of 894nt, matrix protein (M) gene of 609nt, glycoprotein (G) gene of 1575nt...

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Bibliographic Details
Published in:Virus research 2008-08, Vol.135 (2), p.260-266
Main Authors: Du, Jialiang, Zhang, Qiang, Tang, Qing, Li, Hao, Tao, Xiaoyan, Morimoto, Kinjiro, Nadin-Davis, Susan A., Liang, Guodong
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Characterization
China
CTN181
DNA Primers
DNA-Directed RNA Polymerases - chemistry
DNA-Directed RNA Polymerases - genetics
Genome, Viral
Humans
Molecular Sequence Data
Phylogeny
Rabies Vaccines
Rabies virus
Rabies virus - classification
Rabies virus - genetics
Reference Standards
Sequence Analysis, DNA
Viral Proteins - chemistry
Viral Proteins - genetics
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Summary:Human rabies virus vaccine strain CTN181 from China was sequenced. The overall length of the genome was 11,923 nucleotides (nt), comprising a leader sequence of 58nt, nucleoprotein (N) gene of 1353nt, phosphoprotein (P) gene of 894nt, matrix protein (M) gene of 609nt, glycoprotein (G) gene of 1575nt, RNA-dependent RNA polymerase (RdRp, L) gene of 6387nt, and a trailer region of 70nt. The five monocistrons are separated by intergenic regions (IGRs) of 2, 5, 5 and 24 nucleotides (nt), respectively. Two obvious differences between CTN181 and the other rabies virus vaccine strains were (1) the putative stop/polyadenylation signal of the G gene has only one poly (A) tract for CTN181, and (2) the start of the open reading frame for L has two repeats of ATG for CTN181. Both were similar to the SHBRV-18 (silver-haired bat-associated RV strain 18) strain. In addition, some mutations and new functional regions were discovered that are presumed crucial to the function of leader region and L protein. There is an equal role for all five genes in the phylogenetics of rabies virus.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2008.04.002