origin of the C-genome and cytoplasm of Avena polyploids

The contribution of C-genome diploid species to the evolution of polyploid oats was studied using C-genome ITS-specific primers. SCAR analysis among Avena accessions confirmed the presence of C-genome ITS1-5.8S-ITS2 sequences in the genome of AACC and AACCDD polyploids. In situ hybridization and scr...

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Bibliographic Details
Published in:Theoretical and applied genetics 2008-07, Vol.117 (2), p.273-281
Main Authors: Nikoloudakis, N, Katsiotis, A
Format: Article
Language:English
Subjects:
Agriculture
Avena - genetics
Base Sequence
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Classical genetics, quantitative genetics, hybrids
Cloning
Cytoplasm - genetics
DNA, Intergenic - genetics
Evolution
Fundamental and applied biological sciences. Psychology
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Genome, Plant - genetics
Genomes
Introns - genetics
Life Sciences
Molecular Sequence Data
Oats
Original Paper
Phylogenetics
Phylogeny
Physical Chromosome Mapping
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Polymorphism, Genetic
Polyploidy
Pteridophyta, spermatophyta
RNA, Ribosomal, 18S - genetics
Vegetals
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Summary:The contribution of C-genome diploid species to the evolution of polyploid oats was studied using C-genome ITS-specific primers. SCAR analysis among Avena accessions confirmed the presence of C-genome ITS1-5.8S-ITS2 sequences in the genome of AACC and AACCDD polyploids. In situ hybridization and screening of more than a thousand rRNA clones in Avena polyploid species containing the C-genome revealed substantial C-genome rRNA sequence elimination. C-genome clones sequenced and Maximum Likelihood Parsimony analysis revealed close proximity to Avena ventricosa ITS1-5.8S-ITS2 sequences, providing strong evidence of the latter's active role in the evolution of tetraploid and hexaploid oats. In addition, cloning and sequencing of the chloroplastic trnL intron among the most representative Avena species verified the maternal origin of A-genome for the AACC interspecific hybrid formation, which was the genetic bridge for the establishment of cultivated hexaploid oats.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-008-0772-9