Development of solid-phase chemiluminescence immunoassays for digoxin comparing flow injection and sequential injection techniques

The development of a competitive solid-phase immunoassay for digoxin making use of the acridinium chemiluminescence system is described. Two different instrumental approaches are compared. One is based on a continuous flow system using a peristaltic flow injection analysis pump; the other uses a new...

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Bibliographic Details
Published in:Analyst (London) 1998-11, Vol.123 (11), p.2271-2276
Main Authors: DREVENY, D, MICHALOWSKI, J, SEIDL, R, GÜBITZ, G
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Biotechnology
Cardiotonic Agents - analysis
Cardiotonic Agents - chemistry
Chemistry
Digoxin - analysis
Digoxin - chemistry
Exact sciences and technology
Flow Injection Analysis
Fundamental and applied biological sciences. Psychology
General, instrumentation
Immunoenzyme Techniques - instrumentation
Luminescent Measurements
Methods. Procedures. Technologies
Others
Various methods and equipments
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Description
Summary:The development of a competitive solid-phase immunoassay for digoxin making use of the acridinium chemiluminescence system is described. Two different instrumental approaches are compared. One is based on a continuous flow system using a peristaltic flow injection analysis pump; the other uses a new sequential injection technique. In both systems a flow cell, consisting of transparent PTFE tubing packed with immobilized antibodies, acts as an immunoreactor. The entire assay, including both the immunoreaction and the chemiluminescence reaction, takes place in this immunoreactor cell. Compared with the flow injection technique, the sequential injection mode showed higher precision, ranging from 2.16 to 5.5% RSD depending on concentration. The total assay time, including regeneration, is less than 8 min with the sequential injection technique. The detection limit for both techniques is in the low femtomole range.
ISSN:0003-2654
1364-5528
DOI:10.1039/a804182b