Colonization of maternal and fetal tissues by Porphyromonas gingivalis is strain-dependent in a rodent animal model

Objective The objective of this study was to develop a rodent model of Porphyromonas gingivalis infection during pregnancy. Study Design Sprague Dawley rats were infected intravenously with 105 , 107 , or 109 CFU per dam of P gingivalis strain W83, ATCC 33277, or A7436 at gestational day 14 and necr...

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Published in:American journal of obstetrics and gynecology 2008-07, Vol.199 (1), p.86.e1-86.e7
Main Authors: Bélanger, Myriam, PhD, Reyes, Leticia, DVM, PhD, von Deneen, Karen, DVM, Reinhard, Mary K., DVM, Progulske-Fox, Ann, PhD, Brown, Mary B., PhD
Format: Article
Language:English
Subjects:
Animals
Bacteroidaceae Infections - transmission
Biological and medical sciences
Chorioamnionitis - microbiology
Disease Models, Animal
Female
Gynecology. Andrology. Obstetrics
Infectious Disease Transmission, Vertical
Medical sciences
Obstetrics and Gynecology
periodontal disease
Placenta - microbiology
Porphyromonas gingivalis
Porphyromonas gingivalis - isolation & purification
Pregnancy
Rats
Rats, Sprague-Dawley
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Summary:Objective The objective of this study was to develop a rodent model of Porphyromonas gingivalis infection during pregnancy. Study Design Sprague Dawley rats were infected intravenously with 105 , 107 , or 109 CFU per dam of P gingivalis strain W83, ATCC 33277, or A7436 at gestational day 14 and necropsied at gestational day 18. Maternal organs were cultured to assess the spread of the infection. Six fetal units (placenta, amniotic fluid, membranes, and fetus) per dam were cultured; additional fetal units were examined by histopathology. Polymerase chain reaction was performed on placentas. Results Colonization rates were dependent on the strain of P gingivalis used and the infection dose. At an infection dose of 109 CFU/dam, P gingivalis W83, ATCC 33277, or A7436 was detected in 33%, 83%, or 100% of placentas, respectively. Epithelial hyperplasia, cellular necrosis, and inflammatory infiltrate were observed in infected placental tissues. Conclusion This study demonstrated that P gingivalis can invade both maternal and fetal tissues, resulting in chorioamnionitis and placentitis.
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2007.11.067