Rapid Photometric Assay Evaluating Antioxidative Activity in Edible Plant Material

• Published in: Journal of agricultural and food chemistry 1999-08, Vol.47 (8), p.3206-3209
• Main Authors: Kuo, Jen-Min, Yeh, Dong-Bor, Pan, Bonnie Sun
• Format: Article
• Language: English
• Subjects: antioxidants; Antioxidants - analysis; Antioxidants - isolation & purification; Antioxidants - pharmacology; assays; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Food industries; food plants; Fundamental and applied biological sciences. Psychology; General aspects; General pharmacology; hemoglobin; Hemoglobins - drug effects; Kinetics; Linoleic Acid; lipid peroxidation; Lipid Peroxidation - drug effects; Magnoliopsida - chemistry; Medical sciences; Methods of analysis, processing and quality control, regulation, standards; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; photometry; Plant Extracts - chemistry; Plants, Edible - chemistry; rapid methods; Research and development. New food products, dietetic foods and beverages; Spectrophotometry - methods; Spices; Tea - chemistry; Vegetables - chemistry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf981351o

The objective of the present study is to develop a rapid and convenient method to determine antioxidative activity. It was determined by the inhibition capacity on the hemoglobin-catalyzed peroxidation of linoleic acid. The appropriate conditions for reaction of 4 mM linoleic acid were 0.002% hemoglobin at 37 °C for 10 min. Adding methanol to the reaction mixture at