Monocyte Rescue of Human T Cells from Apoptosis is CD40/CD154 Dependent

The induction of T‐cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T‐cell apoptosis did not require autologous monocytes. Inhibition required direct contact with mon...

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Bibliographic Details
Published in:Scandinavian journal of immunology 1999-11, Vol.50 (5), p.479-484
Main Authors: Sakata, K, Sakata, A, Kong, L, Vela-Roch, N, Talal, N, Dang, H
Format: Article
Language:English
Subjects:
actinomycin D
Antibodies, Blocking - pharmacology
Apoptosis - immunology
CD154 antigen
CD40 antigen
CD40 Antigens - metabolism
CD40 Ligand
cycloheximide
Cycloheximide - pharmacology
Dactinomycin - pharmacology
FasL protein
Formaldehyde - pharmacology
Humans
In Vitro Techniques
Ligands
Lipopolysaccharide Receptors - metabolism
Lupus Erythematosus, Systemic - immunology
Lupus Erythematosus, Systemic - pathology
Lymphocyte Activation
Membrane Glycoproteins - metabolism
Monocytes - drug effects
Monocytes - immunology
paraformaldehyde
Polymers - pharmacology
T-Lymphocytes - cytology
T-Lymphocytes - immunology
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Summary:The induction of T‐cell apoptosis is regulated in part by monocytes (CD14+ cells). Human peripheral blood monocytes inhibited the spontaneous cell death of activated T cells in vitro. The inhibition of T‐cell apoptosis did not require autologous monocytes. Inhibition required direct contact with monocytes and was not due to a soluble factor. Furthermore, treatment of monocytes with actinomycin D, cycloheximide and paraformaldehyde abrogated the anti‐apoptotic activity of these cells. Blocking antibody to CD40 and CD154 (CD40 ligand) decreased the ability of monocytes to aid in T‐cell survival, whereas, blocking LFA‐1/I‐CAM‐1, Fas ligand and the CD4/major histocompatibility complex class II interaction did not affect the influence of monocytes on T‐cell survival. This shows that monocytes rescue of activated T cells from apoptosis is dependent upon CD40/CD154 interaction.
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.1999.00629.x