Using the Acetyl Bromide Assay To Determine Lignin Concentrations in Herbaceous Plants:  Some Cautionary Notes

The acetyl bromide assay was developed to provide a rapid and sensitive method for quantifying lignin in woody plant species. The original procedure cautioned against prolonged reaction times and advised keeping the reaction temperature at 70 °C to prevent excessive carbohydrate degradation that wou...

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Published in:Journal of agricultural and food chemistry 1999-02, Vol.47 (2), p.628-632
Main Authors: Hatfield, R. D, Grabber, J, Ralph, J, Brei, K
Format: Article
Language:English
Subjects:
Acetates - chemistry
alfalfa
assays
Biological and medical sciences
Cell Wall - chemistry
cell wall components
Chemical constitution
corn
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
lignin
Lignin - analysis
Medicago sativa - chemistry
Plant physiology and development
Plants - chemistry
quantitative analysis
rapid methods
Space life sciences
xylan
Zea mays - chemistry
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cited_by cdi_FETCH-LOGICAL-a468t-96962f552e9b1ce4105ff680ebd9c82d62d24c867747dddc25ddda8f4513c69c3
cites cdi_FETCH-LOGICAL-a468t-96962f552e9b1ce4105ff680ebd9c82d62d24c867747dddc25ddda8f4513c69c3
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container_title Journal of agricultural and food chemistry
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creator Hatfield, R. D
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Ralph, J
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description The acetyl bromide assay was developed to provide a rapid and sensitive method for quantifying lignin in woody plant species. The original procedure cautioned against prolonged reaction times and advised keeping the reaction temperature at 70 °C to prevent excessive carbohydrate degradation that would skew the absorption spectra. Characterization of the reaction conditions revealed that the acetyl bromide reagent readily degrades xylans, a prominent polysaccharide group within all lignified plants. This degradation results in increased absorbance in the 270−280 nm region that is used to quantify lignin. The degradation of xylans is temperature dependent and is exacerbated by the addition of perchloric acid. Lowering the reaction temperature to 50 °C and increasing the reaction time from 2 to 4 h allows complete lignin solubilization but minimizes degradation of the xylans. Keywords: Acetyl bromide lignin assay; lignin; cell wall; xylans; alfalfa; corn
doi_str_mv 10.1021/jf9808776
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Acetates - chemistry
alfalfa
assays
Biological and medical sciences
Cell Wall - chemistry
cell wall components
Chemical constitution
corn
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
Indicators and Reagents
lignin
Lignin - analysis
Medicago sativa - chemistry
Plant physiology and development
Plants - chemistry
quantitative analysis
rapid methods
Space life sciences
xylan
Zea mays - chemistry
title Using the Acetyl Bromide Assay To Determine Lignin Concentrations in Herbaceous Plants:  Some Cautionary Notes
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