Quantitative HPLC Determination of the Antioxidant Activity of Capsaicin on the Formation of Lipid Hydroperoxides of Linoleic Acid:  A Comparative Study against BHT and Melatonin

The antioxidant activity of capsaicin, as compared to BHT and melatonin, was determined by the direct measurement of lipid hydroperoxides formed upon linoleic acid autoxidation initiated by AIBN. The formation of four isomeric lipid hydroperoxides was detected after reverse-phase HPLC separation. Da...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-07, Vol.47 (7), p.2563-2570
Main Authors: Henderson, David E, Slickman, Adam M, Henderson, Susan K
Format: Article
Language:English
Subjects:
antioxidants
Antioxidants - chemistry
Antioxidants - pharmacology
autoxidation
Biological and medical sciences
butylated hydroxytoluene
Butylated Hydroxytoluene - pharmacology
capsaicin
Capsaicin - chemistry
Capsaicin - pharmacology
Chromatography, High Pressure Liquid - methods
comparisons
Food additives
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
linoleic acid
Linoleic Acid - chemistry
lipid peroxides
Lipid Peroxides - chemical synthesis
melatonin
Melatonin - pharmacology
oxidation
quantitative analysis
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Summary:The antioxidant activity of capsaicin, as compared to BHT and melatonin, was determined by the direct measurement of lipid hydroperoxides formed upon linoleic acid autoxidation initiated by AIBN. The formation of four isomeric lipid hydroperoxides was detected after reverse-phase HPLC separation. Data from three detectors, UV absorption, glassy carbon electrode electrochemical detection, and postcolumn chemiluminescence using luminol, were compared. Capsaicin was more effective than melatonin in suppressing the formation of lipid hydroperoxides but not as effective as BHT. The formation of capsaicin and BHT dimers was observed during oxidation, and the dimers were characterized using APCI MSn. Keywords: Lipid hydroperoxide; capsaicin; butylated hydroxytoluene; melatonin; electrochemical detection; HPLC; APCI-MS; antioxidant; linoleic acid autoxidation
ISSN:0021-8561
1520-5118
DOI:10.1021/jf980949t