Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis

The random amplified polymorphic DNA (RAPD) technique was employed in the haplo‐diploid dioecious species Gracilaria gracilis to identify sex‐linked PCR markers. Sixty‐nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid fe...

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Bibliographic Details
Published in:Molecular ecology 1999-09, Vol.8 (9), p.1533-1538
Main Authors: Martinez, E.A, Destombe, C, Quillet, M.C, Valero, M
Format: Article
Language:English
Subjects:
Base Sequence
BSA
Diploidy
DNA
DNA Primers
females
Genetic Markers
Gracilaria gracilis
haplo-diploidy
Haploidy
males
Marine
Molecular Sequence Data
nucleotide sequences
Phenotype
Random Amplified Polymorphic DNA Technique
RAPD
Rhodophycota
Rhodophyta
Rhodophyta - genetics
Rhodophyta - physiology
Sequence Analysis, DNA
sex determination
sex linkage
sex-linked markers
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Summary:The random amplified polymorphic DNA (RAPD) technique was employed in the haplo‐diploid dioecious species Gracilaria gracilis to identify sex‐linked PCR markers. Sixty‐nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430‐bp fragment specific to males and a 620‐bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co‐occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploid individual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294x.1999.00721.x