Quantitative Analysis of the 2002 Phocine Distemper Epidemic in The Netherlands

• Published in: Veterinary pathology 2008-07, Vol.45 (4), p.516-530
• Main Authors: Rijks, J.M, Read, F.L, Bildt, M.W.G. van de, Bolhuis, H.G. van, Martina, B.E.E, Wagenaar, J.A, Meulen, K. van der, Osterhaus, A.D.M.E, Kuiken, T
• Format: Article
• Language: English
• Subjects: Age Factors; Animals; Antigens, Viral - analysis; Disease Outbreaks - veterinary; Distemper - epidemiology; Distemper - virology; Distemper Virus, Phocine - genetics; Distemper Virus, Phocine - isolation & purification; Enzyme-Linked Immunosorbent Assay - veterinary; Immunoglobulin M - blood; Immunohistochemistry - veterinary; Netherlands - epidemiology; Phoca - virology; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Viral - chemistry; RNA, Viral - genetics
• ISSN: 0300-9858; 1544-2217
• DOI: 10.1354/vp.45-4-516

Phocine distemper virus (PDV) caused thousands of deaths among harbor seals (Phoca vitulina) from the North Sea in 1988 and 2002. To examine the effects of different factors on the pathology of phocine distemper, we performed necropsies and laboratory analyses on 369 harbor seals that stranded along the Dutch coast during the 2002 PDV epidemic. Diagnostic tests for morbillivirus infection indicated a differential temporal presence of morbillivirus in lung and brain. Seals of 3 years or older were significantly more often IgG positive than younger seals. The most frequent lesions in PDV cases were bronchopneumonia, broncho-interstitial pneumonia, and interstitial emphysema. Extra-thoracic emphysema was rare in