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Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium
Large conductance voltage-dependent and Ca 2+-modulated K + channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent...
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| Published in: | FEBS letters 1999-11, Vol.460 (3), p.427-432 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4260-4b8fe49bed934114b9fa7ccf51aae8784027ad0692d5675a5e156f517d292d623 |
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| cites | cdi_FETCH-LOGICAL-c4260-4b8fe49bed934114b9fa7ccf51aae8784027ad0692d5675a5e156f517d292d623 |
| container_end_page | 432 |
| container_issue | 3 |
| container_start_page | 427 |
| container_title | FEBS letters |
| container_volume | 460 |
| creator | Song, Min Zhu, Ning Olcese, Riccardo Barila, Bruna Toro, Ligia Stefani, Enrico |
| description | Large conductance voltage-dependent and Ca
2+-modulated K
+ channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na
+/K
+-ATPase protein and RNA levels do not significantly change at different stages of pregnancy. The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition. |
| doi_str_mv | 10.1016/S0014-5793(99)01394-0 |
| format | article |
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2+-modulated K
+ channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na
+/K
+-ATPase protein and RNA levels do not significantly change at different stages of pregnancy. The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)01394-0</identifier><identifier>PMID: 10556510</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Blotting, Western ; Cells, Cultured ; COS Cells ; Female ; Hormone ; hSlo, human MaxiK ; IbTx, iberiotoxin ; K channel ; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits ; Large-Conductance Calcium-Activated Potassium Channels ; MaxiK, large conductance voltage-dependent and calcium-activated K+ channel ; Membrane Potentials ; mRNA ; Myometrium ; Myometrium - cytology ; Myometrium - metabolism ; PAGE, polyacrylamide gel electrophoresis ; PBS, phosphate-buffered saline ; Placental Hormones - physiology ; Potassium Channels - biosynthesis ; Potassium Channels - genetics ; Potassium Channels - physiology ; Potassium Channels, Calcium-Activated ; Pregnancy ; Protein expression ; Rats ; Rats, Sprague-Dawley ; Ribonucleases - metabolism ; RNA, Messenger - biosynthesis ; RNA, Messenger - metabolism ; Sodium-Potassium-Exchanging ATPase - metabolism ; TBS, Tris-buffered saline ; Time Factors</subject><ispartof>FEBS letters, 1999-11, Vol.460 (3), p.427-432</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 460 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4260-4b8fe49bed934114b9fa7ccf51aae8784027ad0692d5675a5e156f517d292d623</citedby><cites>FETCH-LOGICAL-c4260-4b8fe49bed934114b9fa7ccf51aae8784027ad0692d5675a5e156f517d292d623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579399013940$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10556510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Min</creatorcontrib><creatorcontrib>Zhu, Ning</creatorcontrib><creatorcontrib>Olcese, Riccardo</creatorcontrib><creatorcontrib>Barila, Bruna</creatorcontrib><creatorcontrib>Toro, Ligia</creatorcontrib><creatorcontrib>Stefani, Enrico</creatorcontrib><title>Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Large conductance voltage-dependent and Ca
2+-modulated K
+ channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na
+/K
+-ATPase protein and RNA levels do not significantly change at different stages of pregnancy. The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>COS Cells</subject><subject>Female</subject><subject>Hormone</subject><subject>hSlo, human MaxiK</subject><subject>IbTx, iberiotoxin</subject><subject>K channel</subject><subject>Large-Conductance Calcium-Activated Potassium Channel alpha Subunits</subject><subject>Large-Conductance Calcium-Activated Potassium Channels</subject><subject>MaxiK, large conductance voltage-dependent and calcium-activated K+ channel</subject><subject>Membrane Potentials</subject><subject>mRNA</subject><subject>Myometrium</subject><subject>Myometrium - cytology</subject><subject>Myometrium - metabolism</subject><subject>PAGE, polyacrylamide gel electrophoresis</subject><subject>PBS, phosphate-buffered saline</subject><subject>Placental Hormones - physiology</subject><subject>Potassium Channels - biosynthesis</subject><subject>Potassium Channels - genetics</subject><subject>Potassium Channels - physiology</subject><subject>Potassium Channels, Calcium-Activated</subject><subject>Pregnancy</subject><subject>Protein expression</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Ribonucleases - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>TBS, Tris-buffered saline</subject><subject>Time Factors</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqNkMFu1DAURa0K1A6ln1DkFYJFqJ3YSbxCpWoZRAEJ2rXl2C-qkWMPdlI6n9Uf6TfVmVSIHawsv3vuffZF6JiSd5TQ-uQHIZQVvBHVGyHeEloJVpA9tKJtUxUVq9tnaPUHOUAvUvpJ8r2lYh8dUMJ5zSlZIbMOcQheOayDH2NwOPR4E8MI1mO420RIyQaPlTd4-P71FDu4BZdmarwB_EXd2c9Y3yjvweGHe5ymbvJ2xNk9bMMAY7TT8BI975VLcPR0HqLri_Ors3Vx-e3jp7PTy0KzsiYF69oemOjAiIpRyjrRq0brnlOloG1aRspGGVKL0vC64YoD5XVWG1PmUV1Wh-j1kps_8GuCNMrBJg3OKQ9hSjI7G1bxGeQLqGNIKUIvN9EOKm4lJXKuV-7qlXN3Ugi5q1eS7Hv1tGDqBjB_uZY-M7BegN_Wwfb_UuXF-Ydyp8yCELvxHPV-icp1w62FKJO24DUYG0GP0gT7j9c-Ao31nuA</recordid><startdate>19991105</startdate><enddate>19991105</enddate><creator>Song, Min</creator><creator>Zhu, Ning</creator><creator>Olcese, Riccardo</creator><creator>Barila, Bruna</creator><creator>Toro, Ligia</creator><creator>Stefani, Enrico</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991105</creationdate><title>Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium</title><author>Song, Min ; Zhu, Ning ; Olcese, Riccardo ; Barila, Bruna ; Toro, Ligia ; Stefani, Enrico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4260-4b8fe49bed934114b9fa7ccf51aae8784027ad0692d5675a5e156f517d292d623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cells, Cultured</topic><topic>COS Cells</topic><topic>Female</topic><topic>Hormone</topic><topic>hSlo, human MaxiK</topic><topic>IbTx, iberiotoxin</topic><topic>K channel</topic><topic>Large-Conductance Calcium-Activated Potassium Channel alpha Subunits</topic><topic>Large-Conductance Calcium-Activated Potassium Channels</topic><topic>MaxiK, large conductance voltage-dependent and calcium-activated K+ channel</topic><topic>Membrane Potentials</topic><topic>mRNA</topic><topic>Myometrium</topic><topic>Myometrium - cytology</topic><topic>Myometrium - metabolism</topic><topic>PAGE, polyacrylamide gel electrophoresis</topic><topic>PBS, phosphate-buffered saline</topic><topic>Placental Hormones - physiology</topic><topic>Potassium Channels - biosynthesis</topic><topic>Potassium Channels - genetics</topic><topic>Potassium Channels - physiology</topic><topic>Potassium Channels, Calcium-Activated</topic><topic>Pregnancy</topic><topic>Protein expression</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Ribonucleases - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>TBS, Tris-buffered saline</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Min</creatorcontrib><creatorcontrib>Zhu, Ning</creatorcontrib><creatorcontrib>Olcese, Riccardo</creatorcontrib><creatorcontrib>Barila, Bruna</creatorcontrib><creatorcontrib>Toro, Ligia</creatorcontrib><creatorcontrib>Stefani, Enrico</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Min</au><au>Zhu, Ning</au><au>Olcese, Riccardo</au><au>Barila, Bruna</au><au>Toro, Ligia</au><au>Stefani, Enrico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-11-05</date><risdate>1999</risdate><volume>460</volume><issue>3</issue><spage>427</spage><epage>432</epage><pages>427-432</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Large conductance voltage-dependent and Ca
2+-modulated K
+ channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcriptional regulation of channel expression, mRNA levels quantified with the ribonuclease protection assay correlated well with MaxiK protein levels. As a control, Na
+/K
+-ATPase protein and RNA levels do not significantly change at different stages of pregnancy. The low numbers of MaxiK channels at the end of pregnancy may facilitate uterine contraction needed for parturition.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10556510</pmid><doi>10.1016/S0014-5793(99)01394-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1999-11, Vol.460 (3), p.427-432 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69274352 |
| source | ScienceDirect Journals; Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Blotting, Western Cells, Cultured COS Cells Female Hormone hSlo, human MaxiK IbTx, iberiotoxin K channel Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Large-Conductance Calcium-Activated Potassium Channels MaxiK, large conductance voltage-dependent and calcium-activated K+ channel Membrane Potentials mRNA Myometrium Myometrium - cytology Myometrium - metabolism PAGE, polyacrylamide gel electrophoresis PBS, phosphate-buffered saline Placental Hormones - physiology Potassium Channels - biosynthesis Potassium Channels - genetics Potassium Channels - physiology Potassium Channels, Calcium-Activated Pregnancy Protein expression Rats Rats, Sprague-Dawley Ribonucleases - metabolism RNA, Messenger - biosynthesis RNA, Messenger - metabolism Sodium-Potassium-Exchanging ATPase - metabolism TBS, Tris-buffered saline Time Factors |
| title | Hormonal control of protein expression and mRNA levels of the MaxiK channel α subunit in myometrium |
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