Determination of Solanidine- and Tomatidine-Type Glycoalkaloid Aglycons by Gas Chromatography/Mass Spectrometry

A combined derivatization method for gas chromatographic/mass spectrometric (GC/MS) analysis of steroidal glycoalkaloid (SGA) aglycons was developed using both trimethylsilylation and pentafluoropropionylation. In comparison with underivatized or only silylated aglycons, the new technique produces m...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-07, Vol.47 (7), p.2738-2742
Main Authors: Laurila, Jaana, Laakso, Into, Väänänen, Tiina, Kuronen, Pirjo, Huopalahti, Rainer, Pehu, Eija
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
chemical analysis
determination
Diosgenin
Food industries
Food toxicology
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
gas chromatography
Gas Chromatography-Mass Spectrometry
Genetics and breeding of economic plants
glycoalkaloids
mass spectrometry
monitoring
Reproducibility of Results
single ion monitoring
Solanaceous Alkaloids - analysis
solanidine
Solanum palustre
Solanum tuberosum
steroidal glycoalkaloid aglycons
Tomatine - analogs & derivatives
Tomatine - analysis
Varietal selection. Specialized plant breeding, plant breeding aims
Yield, quality, earliness, varia
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Summary:A combined derivatization method for gas chromatographic/mass spectrometric (GC/MS) analysis of steroidal glycoalkaloid (SGA) aglycons was developed using both trimethylsilylation and pentafluoropropionylation. In comparison with underivatized or only silylated aglycons, the new technique produces more specific and abundant fragmentation for compounds with a tomatidine-type structure. For example, the difference between solasodine and tomatidine, the former containing a double bond at position 5,6 in the steroidal skeleton, can be observed by their base peak fragments at m/z 417 (C24H41O2Si2) and m/z 419 (C24H43O2Si2). The method is well suited for the simultaneous determination of both solanidane- and spirosolane-type SGA aglycons from Solanum species and hybrids. The reproducibility of the method, including SGA extraction, hydrolysis, derivatization, and quantitative GC/MS analysis, was
ISSN:0021-8561
1520-5118
DOI:10.1021/jf981009b