Heterogeneous Helicobacter pylori Isolates from H. pylori—Infected Couples in Taiwan

Helicobacter pylori strain diversity was investigated in 55 H. pylori seropositive couples in Taiwan in biopsy samples from the antrum and corpus. Two DNA typing methods were used to characterize 90 isolates from 25 couples. In only 1 of the 25 couples was the same strain colonized from both partner...

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Bibliographic Details
Published in:The Journal of infectious diseases 1999-12, Vol.180 (6), p.2064-2068
Main Authors: Kuo, Chun-Hsien, Poon, Sek-Kwong, Su, Yi-Chun, Su, Reu, Chang, Chi-Sen, Wang, Wen-Ching
Format: Article
Language:English
Subjects:
Adult
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Biopsies
Concise Communications
DNA
DNA, Bacterial - analysis
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Genetic polymorphism
Genetic Variation
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - classification
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Humans
Infections
Male
Microbiology
Middle Aged
Molecular Sequence Data
Nucleotides
Peptic ulcer
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Spouses
Stomach
Taiwan
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Summary:Helicobacter pylori strain diversity was investigated in 55 H. pylori seropositive couples in Taiwan in biopsy samples from the antrum and corpus. Two DNA typing methods were used to characterize 90 isolates from 25 couples. In only 1 of the 25 couples was the same strain colonized from both partners. Comparison of isolates from 2 sites in each of 40 patients showed that 9 pairs were distinct but might be related. Peptic ulcer occurred in 77.8% of these 9 patients compared with 29% of 31 patients with the same predominant strain in 2 biopsies (P = .025). Random amplified polymorphic DNA and sequence analyses of 2 closely related isolates from 1 patient support the hypothesis that development of genetic diversity of H. pylori results from horizontal genetic exchange during long-term colonization of mixed bacterial populations.
ISSN:0022-1899
1537-6613
DOI:10.1086/315130