The endothelial nitric oxide synthase Glu298Asp polymorphism is not a risk factor for endometriosis in south Indian women

Abstract Objective To investigate whether the eNOS gene influences the risk of developing endometriosis in south Indian women. Study design The single nucleotide polymorphism, Glu298Asp, in exon7 of the eNOS gene was tested for association in a case–control study of 232 affected women and 210 women...

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Published in:European journal of obstetrics & gynecology and reproductive biology 2008-07, Vol.139 (1), p.53-58
Main Authors: Bhanoori, Manjula, Kameshwari, D.B, Zondervan, K.T, Deenadayal, Mamata, Kennedy, Stephen, Shivaji, S
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Case-Control Studies
Cohort Studies
Endometriosis
Endometriosis - genetics
Endometriosis - metabolism
Endometrium - metabolism
eNOS
Female
Female genital diseases
Genetic Predisposition to Disease - genetics
Gynecology. Andrology. Obstetrics
Humans
India
Medical sciences
Nitric Oxide Synthase Type III - genetics
Nitric Oxide Synthase Type III - metabolism
Non tumoral diseases
Obstetrics and Gynecology
Polymorphism
Polymorphism, Single Nucleotide - genetics
Young Adult
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Summary:Abstract Objective To investigate whether the eNOS gene influences the risk of developing endometriosis in south Indian women. Study design The single nucleotide polymorphism, Glu298Asp, in exon7 of the eNOS gene was tested for association in a case–control study of 232 affected women and 210 women with no evidence of disease. All the women were infertile, ascertained from the same infertility clinic. The genotype frequencies of the polymorphism were compared, using polymerase chain reaction and sequencing analysis. The localization and expression of eNOS in the eutopic endometrium of five cases and four controls was also analyzed using immunohistochemistry and western blotting. Results No statistically significant differences were observed in the genotype distributions and allele frequencies ( p = 0.3) between the cases and controls according to codominant, dominant and recessive genotype models. The localization and expression of this protein were similar in the endometrium of cases and controls. Conclusion In the present study we could neither observe a difference in the eNOS expression nor establish an association between the eNOS Glu298Asp exon 7 polymorphism in south Indian women with endometriosis.
ISSN:0301-2115
1872-7654
DOI:10.1016/j.ejogrb.2008.01.006