Fluorescence multicolor hologram recorded by using a macrolens array

We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using...

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Bibliographic Details
Published in:Optics letters 2008-07, Vol.33 (13), p.1461-1463
Main Authors: SHAKED, Natan T, KATZ, Barak, ROSEN, Joseph
Format: Article
Language:English
Subjects:
Colorimetry - instrumentation
Colorimetry - methods
Computer-generated holograms
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
Fundamental areas of phenomenology (including applications)
Holography
Holography - instrumentation
Image Enhancement - instrumentation
Image forming and processing
Image Interpretation, Computer-Assisted - instrumentation
Imaging and optical processing
Imaging, Three-Dimensional - instrumentation
Lenses
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Optics
Physics
Reproducibility of Results
Sensitivity and Specificity
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Summary:We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using a macrolens array, only nine projections of the 3-D scene are captured in a single camera shot and are then digitally processed to yield the hologram. The 3-D reconstructed images from all the monochromatic holograms are composed into a single multicolor image of the 3-D scene. The proposed holographic technique is demonstrated by experiments.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.33.001461