Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli

• Published in: FEMS microbiology letters 1999-12, Vol.181 (1), p.17-23
• Main Authors: Thaller, Maria Cristina, Schippa, Serena, Bonci, Alessandra, Berlutti, Francesca, Selan, Laura, Rossolini, Gian Maria
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Acid Phosphatase - biosynthesis; Acid Phosphatase - genetics; Adenosine Triphosphatases - genetics; Bacteria; Bacterial Proteins - genetics; Bacteriology; Base Sequence; Biological and medical sciences; Chromosome Mapping; Chromosomes; Cosmids - genetics; DNA-Binding Proteins - genetics; E coli; Enterobacterial chromosome; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli Proteins; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic rearrangement; Genetics; Glucose - metabolism; Homology; Microbiology; Molecular Sequence Data; Phosphatase; Regulation; Restriction Mapping; Salmonella; Salmonella enterica; Salmonella enterica - enzymology; Salmonella enterica - genetics; Salmonella enterica - growth & development; Sequence Alignment; Sequence Analysis, DNA
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1999.tb08821.x

Abstract Unlike in Escherichia coli, in Salmonella enterica production of class B acid phosphatase (AphA) was detectable also in cells growing in the presence of glucose. Characterization of the aphA locus from a S. enterica ser. typhi strain showed that the aphA determinant is very similar to the E. coli homolog, and that its chromosomal location between the highly conserved tyrB and uvrA genes is retained. However, the aphA flanking regions were found to be markedly different in the two species, either between tyrB and aphA or between aphA and uvrA. The differences in the aphA 5′-flanking region, which in S. enterica is considerably shorter than in E. coli (183 vs. 1121 bp) and includes potential promoter sequences not present in E. coli, could be responsible for the different regulation of class B acid phosphatase observed in the two species.