Analysis of Food Oligosaccharides Using MALDI-MS:  Quantification of Fructooligosaccharides

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful new technique that will have a great impact on food analysis. This study demonstrates the applicability of MALDI-MS performed directly on an aqueous food extract for qualitative and quantitative analysis of food o...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-04, Vol.47 (4), p.1549-1557
Main Authors: Wang, Jian, Sporns, Peter, Low, Nicholas H
Format: Article
Language:English
Subjects:
Allium ampeloprasum
Biological and medical sciences
Biotechnology
bulbous vegetables
Carbohydrate Conformation
Carbohydrate Sequence
Cyclodextrins - chemistry
dextrins
elephant garlic
Food Analysis
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
gamma-cyclodextrin
gamma-Cyclodextrins
General aspects
General pharmacology
high performance anion exchange chromatography
Indicators and Reagents
inulin
ion exchange chromatography
Jerusalem artichokes
maltohexaose
mass spectrometry
matrix-assisted laser desorption/ionization mass spectrometry
Medical sciences
Methods of analysis, processing and quality control, regulation, standards
Molecular Sequence Data
oligosaccharides
Oligosaccharides - analysis
Oligosaccharides - chemistry
onions
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
shallots
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful new technique that will have a great impact on food analysis. This study demonstrates the applicability of MALDI-MS performed directly on an aqueous food extract for qualitative and quantitative analysis of food oligosaccharides. 2‘,4‘,6‘-Trihydroxyacetophenone was found to be the best matrix for analysis of oligosaccharides in the foods examined. The relationship between laser strength, resolution, and the response factors of individual oligosaccharides using MALDI-MS was investigated. A MALDI-MS method for quantitative analysis of fructooligosaccharides with standard addition of a pure fructooligosaccharide was developed. High performance anion exchange chromatography with pulsed amperometric detection was compared to MALDI-MS for the analysis of fructooligosaccharides. The fructooligosaccharide analyses were performed on red onions, shallots, and elephant garlic. Keywords: Cyclodextrin; maltohexaose; fructan; inulin; kestose; nystose
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9809380