Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time fo...

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Bibliographic Details
Published in:Gene 1999-10, Vol.238 (2), p.375-385
Main Authors: Schummer, Michèl, Ng, WaiLap V, Bumgarner, Roger E, Nelson, Peter S, Schummer, Bernhard, Bednarski, David W, Hassell, Laurie, Baldwin, Rae Lynn, Karlan, Beth Y, Hood, Leroy
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - genetics
Cancer maker
Cells, Cultured
Clone Cells
Differential expression
DNA array
DNA microarrays
DNA, Complementary
Female
HE4
HE4 gene
Humans
Nucleic Acid Hybridization
Ovarian Neoplasms - genetics
Ovary - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Sensitivity and Specificity
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Summary:Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21 500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(99)00342-X