Autophagy Is Essential for Preimplantation Development of Mouse Embryos

After fertilization, maternal proteins in oocytes are degraded and new proteins encoded by the zygotic genome are synthesized. We found that autophagy, a process for the degradation of cytoplasmic constituents in the lysosome, plays a critical role during this period. Autophagy was triggered by fert...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2008-07, Vol.321 (5885), p.117-120
Main Authors: Tsukamoto, Satoshi, Kuma, Akiko, Murakami, Mirei, Kishi, Chieko, Yamamoto, Akitsugu, Mizushima, Noboru
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Autophagy
Autophagy-Related Protein 5
Biological and medical sciences
Blastocyst
Blastocyst - physiology
Cells, Cultured
Cellular biology
Embryology
Embryology: invertebrates and vertebrates. Teratology
Embryonic Development
Embryos
Female
Female animals
Fertilization
Fundamental and applied biological sciences. Psychology
Genomics
Lysosomes - physiology
Lysosomes - ultrastructure
Male
Male animals
Mating behavior
Mice
Mice, Knockout
Mice, Transgenic
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Oocytes
Oocytes - physiology
Organogenesis. Fetal development
Organogenesis. Physiological fonctions
Parthenogenesis
Phagosomes - physiology
Phagosomes - ultrastructure
Pregnancy
Protein Biosynthesis
Protein synthesis
Proteins - metabolism
Recombinant Fusion Proteins - metabolism
Rodents
Spermatozoa
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Summary:After fertilization, maternal proteins in oocytes are degraded and new proteins encoded by the zygotic genome are synthesized. We found that autophagy, a process for the degradation of cytoplasmic constituents in the lysosome, plays a critical role during this period. Autophagy was triggered by fertilization and up-regulated in early mouse embryos. Autophagy-defective oocytes derived from oocyte-specific Atg5 (autophagy-related 5) knockout mice failed to develop beyond the four- and eight-cell stages if they were fertilized by Atg5-null sperm, but could develop if they were fertilized by wild-type sperm. Protein synthesis rates were reduced in the autophagy-null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1154822