Male Mice Deficient for Germ-Cell Cyritestin Are Infertile

Cyritestin is a membrane-anchored sperm protein belonging to the ADAM ( isintegrin and etalloprotease) family of proteins, which are proposed to be involved in cell-cell adhesion through binding to integrin receptors. Several lines of evidence support a role of cyritestin and other members of this p...

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Bibliographic Details
Published in:Biology of reproduction 1999-12, Vol.61 (6), p.1445-1451
Main Authors: SHAMSADIN, R, ADHAM, I. M, NAYERNIA, K, HEINLEIN, U. A. O, OBERWINKLER, H, ENGEL, W
Format: Article
Language:English
Subjects:
ADAM Proteins
Animals
Biological and medical sciences
Cell Line
Chimera
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Homozygote
Infertility, Male - etiology
Male
Mammalian male genital system
Membrane Glycoproteins - deficiency
Membrane Glycoproteins - genetics
Membrane Glycoproteins - physiology
Metalloendopeptidases - deficiency
Metalloendopeptidases - genetics
Metalloendopeptidases - physiology
Mice
Mice, Mutant Strains
Morphology. Physiology
Mutation
Sperm-Ovum Interactions
Spermatozoa - physiology
Vertebrates: reproduction
Zona Pellucida - physiology
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Summary:Cyritestin is a membrane-anchored sperm protein belonging to the ADAM ( isintegrin and etalloprotease) family of proteins, which are proposed to be involved in cell-cell adhesion through binding to integrin receptors. Several lines of evidence support a role of cyritestin and other members of this protein family in the fusion of sperm and the egg plasma membrane. In an effort to elucidate the physiological function of cyritestin, we have disrupted its locus by homologous recombination. Male homozygous null mutants are infertile, even though spermatogenesis, mating, and migration of sperm from the uterus into the oviduct are normal. In vitro experiments showed that infertility is due to the inability of the cyritestin-deficient sperm to bind to the zona pellucida. However, after removal of the zona pellucida, sperm-egg membrane fusion monitored by the presence of pronuclei and generation of 2- and 4-cell embryos did not reveal any differences from the wild-type situation. These results demonstrate that cyritestin is crucial in the fertilization process at the level of the sperm-zona pellucida interaction.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod61.6.1445