TRARESA: a tissue microarray-based hospital system for biomarker validation and discovery

Formalin fixed and paraffin embedded tissue (FFPE) collections in pathology departments are the largest resource for retrospective biomedical research studies. Based on the literature analysis of FFPE related research, as well as our own technical validation, we present the Translational Research Ar...

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Bibliographic Details
Published in:Pathology 2008-08, Vol.40 (5), p.441-449
Main Authors: Das, Kakoli, Mohd Omar, Mohd Feroz, Ong, Chee Wee, Abdul Rashid, Suhaimi Bin, Peh, Bee Keow, Putti, Thomas Choudary, Tan, Puay Hoon, Chia, Kee Seng, Teh, Ming, Shah, Nilesh, Soong, Richie, Salto-Tellez, Manuel
Format: Article
Language:English
Subjects:
Biomarkers - analysis
DNA - analysis
Formaldehyde
Hospitals
Humans
molecular pathology
Paraffin Embedding
RNA - analysis
Tissue Array Analysis - methods
Tissue Fixation
Tissue microarrays
translational research
Validation Studies as Topic
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Summary:Formalin fixed and paraffin embedded tissue (FFPE) collections in pathology departments are the largest resource for retrospective biomedical research studies. Based on the literature analysis of FFPE related research, as well as our own technical validation, we present the Translational Research Arrays (TRARESA), a tissue microarray centred, hospital based, translational research conceptual framework for both validation and/or discovery of novel biomarkers. TRARESA incorporates the analysis of protein, DNA and RNA in the same samples, correlating with clinical and pathological parameters from each case, and allowing (a) the confirmation of new biomarkers, disease hypotheses and drug targets, and (b) the postulation of novel hypotheses on disease mechanisms and drug targets based on known biomarkers. While presenting TRARESA, we illustrate the use of such a comprehensive approach. The conceptualisation of the role of FFPE-based studies in translational research allows the utilisation of this commodity, and adds to the hypothesis-generating armamentarium of existing high-throughput technologies
ISSN:0031-3025
1465-3931
DOI:10.1080/00313020802198101