detergent-insoluble microdomains, rafts, can be used as an effective immunogen

Detergent-insoluble microdomains, or rafts, act as a platform to transduce signals from the extracellular space into the cytoplasm. In the process of developing monoclonal antibodies against raft molecules for the purpose of studying the molecular mechanism of raft-mediated signaling, we observed th...

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Bibliographic Details
Published in:Glycoconjugate journal 2008-08, Vol.25 (6), p.495-501
Main Authors: Katagiri, Yohko U, Nakajima, Hideki, Sato, Ban, Miyagawa, Yoshitaka, Horiuchi, Yasuomi, Okita, Hajime, Fujimoto, Junichiro, Kiyokawa, Nobutaka
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Antibody
Antigens
Antigens - chemistry
Antigens - immunology
Biochemistry
Biomedical and Life Sciences
Cell Line, Tumor
Chromatography, Thin Layer
Cloning
Detergents - chemistry
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Glycolipids - chemical synthesis
Glycolipids - chemistry
Glycolipids - immunology
Humans
immune response
Immunology
Life Sciences
Membrane Microdomains - chemistry
Membrane Microdomains - immunology
Mice
Molecular biology
Monoglycolipid-specific
Pathology
Raft
Rats
Short Communication
Signal transduction
Solubility
Syngeneic antigen
Toxicity
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Summary:Detergent-insoluble microdomains, or rafts, act as a platform to transduce signals from the extracellular space into the cytoplasm. In the process of developing monoclonal antibodies against raft molecules for the purpose of studying the molecular mechanism of raft-mediated signaling, we observed the uniqueness and certain advantages of immunization with rafts. Simple subcutaneous injection of mice with a phosphate-buffered saline (PBS) suspension of rafts without mixing with Freund's adjuvant made it possible to increase the titer of antiserum reacting with raft components. Interestingly, injection of rafts prepared from certain specific cell lines induced monoglycolipid-specific antibodies. Furthermore, antibodies were produced by raft-immunization of even syngeneic mice. Our findings suggest that this phenomenon does not represent a breakdown of immunological self-tolerance, but typical immune reactions accompanying the class switch from IgM antibodies to IgG antibodies.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-007-9083-7