Adenine Nucleotide Synthesis in Human Erythrocytes Depends on the Mode of Supplementation of Cell Suspension with Adenosine

• Published in: Blood cells, molecules, & diseases molecules, & diseases, 1999-08, Vol.25 (4), p.170-179
• Main Authors: Komarova, Svetlana V, Mosharov, Eugene V, Vitvitsky, Victor M, Ataullakhanov, Fazoil I
• Format: Article
• Language: English
• Subjects: Adenine Nucleotides - biosynthesis; Adenine Nucleotides - blood; Adenosine - pharmacokinetics; Adenosine - pharmacology; Adenosine Triphosphate - analysis; Adenosine Triphosphate - biosynthesis; adenosine, ATP, adenylate pool, energy charge, human erythrocytes; Coformycin - pharmacology; Culture Media, Serum-Free; Dose-Response Relationship, Drug; Erythrocytes - metabolism; Humans; Hypoxanthine - metabolism; Inosine - metabolism; Phosphates - pharmacology; Time Factors
• ISSN: 1079-9796; 1096-0961
• DOI: 10.1006/bcmd.1999.0243

In suspensions of washed human erythrocytes, adenosine added in a single dose to concentrations of 0.1–10.0 mmol/l suspension was deaminated at rates ranging from 10 to 50 mmol/l cells h. The sum of adenosine, inosine, and hypoxanthine concentrations in the suspension, as well as the intracellular concentration of ATP, remained constant. In the presence of 25–50 mmol/l orthophosphate, addition of a single dose of adenosine into erythrocyte suspension increased the ATP concentration by up to 280% of the initial level. If the initial adenosine concentrations were greater than 5 mmol/l suspension, ATP increased independently of adenosine concentration to the level determined only by the concentration of orthophosphate. After orthophosphate was returned to its initial level, ATP in erythrocytes began to decrease. In the presence of coformycin, erythrocytes utilised adenosine at a rate of 0.2–0.3 mmol/l cells h. Their adenylate pool increased at a rate of 0.10–0.16 mmol/l cells h for several hours, but intracellular ATP increased only slightly. The energy charge of cells decreased significantly from 0.86±0.05 (control) to 0.82±0.06. Adenosine continuously pumped into erythrocyte suspensions at rates of 0.02–5.0 mmol/l cells h for several hours caused the adenylate pool of erythrocytes and intracellular ATP to increase synchronously at a rate of 0.02–0.35 mmol/l cells h. The energy charge of these erythrocytes increased significantly up to 0.91±0.03. After pumping of adenosine was stopped, the intracellular ATP and the adenylate pool began to decrease, returning sometimes to the initial level in 2–3 h.