Effects of oestradiol-17beta and testosterone on progesterone production in the cultured granulosa cells of Japanese quail

1. In order to study the effects of steroid hormones on steroidogenesis in the avian ovary, quail granulosa cells were cultured with follicle stimulating hormone (FSH), oestradiol-17beta or testosterone. The progesterone content of the medium during the culture period of 66 h and the following 3 h o...

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Bibliographic Details
Published in:British poultry science 1999-09, Vol.40 (4), p.536-540
Main Authors: Sasanami, T, Mori, M
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
chemical composition
Coturnix
culture media
cultured cells
estradiol
Estradiol - pharmacology
Female
Follicle Stimulating Hormone - pharmacology
follicle-stimulating hormone
granulosa cells
Granulosa Cells - cytology
Granulosa Cells - drug effects
Granulosa Cells - metabolism
Japanese quails
Kinetics
luteinizing hormone
Luteinizing Hormone - pharmacology
ovaries
progesterone
Progesterone - biosynthesis
secretion
Staurosporine - pharmacology
testosterone
Testosterone - pharmacology
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Summary:1. In order to study the effects of steroid hormones on steroidogenesis in the avian ovary, quail granulosa cells were cultured with follicle stimulating hormone (FSH), oestradiol-17beta or testosterone. The progesterone content of the medium during the culture period of 66 h and the following 3 h of incubation with luteinising hormone (LH), was measured by radioimmunoassay. 2. When FSH, oestradiol-17beta or testosterone were added during the 66 h culture, subsequent progesterone production by the cells during 3 h of incubation with LH was significantly increased. 3. However, testosterone also simulated progesterone production in the medium during the 66 h culture period, whereas FSH oroestradiol-17beta did not. 4. Addition of staurosporine during culture inhibited both LH-stimulated progesterone production and testosterone-stimulated progesterone production. 5. These results indicate that the processes during which granulosa cells acquired responsiveness to LH, and testosterone stimulates progesterone production might both be mediated staurosporine-sensitive protein kinase C-dependent pathway in quail granulosa cells.
ISSN:0007-1668
1466-1799